Mycoplasma bovis is a significant bacterial pathogen which is able to persist in cattle and cause chronic diseases. This phenomenon may relate to M. bovis evading the immune system of the host. Immunoglobulin-binding proteins are widely distributed in a variety of pathogenic bacteria, including some Mycoplasma species. These proteins are considered to help the bacteria evade the immune response of the host. Here we found M. bovis strain PG45 can bind to IgG from several animals. MBOVPG45_0375 encodes a putative membrane protein, has strong amino acid sequence similarity with Immunoglobulin G-binding protein in Mycoplasma mycoides subsp. capri. Hence, we constructed recombinant MBOVPG45_0375 (r0375) in the Escherichia coli expression system and demonstrated that r0375 can bind to IgG non-immunologically rather than specific binding similar to interaction of antigen and antibody. Moreover, r0375 can bind to the Fab fragment of IgG. Also, the binding of r0375 and IgG inhibits the formation of antigen-antibody union. Furthermore, MBOVPG45_0376 encodes an IgG-cleaving protein of M. bovis strain PG45. Nevertheless, r0375 binding to IgG is required for the cleavage activity of recombinant 0376 (r0376). The activity of r0376 is also affected by incubation time and temperature. In addition, we found both MBOVPG45_0375 and MBOVPG45_0376 are membrane proteins of M. bovis strain PG45. These results about MBOVPG45_0375 as an IgG-binding protein and MBOVPG45_0376 as an IgG-cleaving protein offer a new insight into the interaction between M. bovis and its host.
机构:
Univ Bordeaux, Inst Europeen Chim & Biol, UMS 3033, F-33607 Pessac, France
SIRIC BRIO, Inst Bergonie, F-33076 Bordeaux, FranceINRA, UMR Biol Fruit & Pathol 1332, F-33882 Villenave Dornon, France
Minder, Laetitia
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Di Primo, Carmelo
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Le Roy, Aline
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h-index: 0
机构:
Univ Grenoble Alpes, Inst Biol Struct, F-38044 Grenoble, France
CNRS, Inst Biol Struct, F-38044 Grenoble, France
CEA, Inst Biol Struct, F-38044 Grenoble, FranceINRA, UMR Biol Fruit & Pathol 1332, F-33882 Villenave Dornon, France
Le Roy, Aline
;
Ebel, Christine
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h-index: 0
机构:
Univ Grenoble Alpes, Inst Biol Struct, F-38044 Grenoble, France
CNRS, Inst Biol Struct, F-38044 Grenoble, France
CEA, Inst Biol Struct, F-38044 Grenoble, FranceINRA, UMR Biol Fruit & Pathol 1332, F-33882 Villenave Dornon, France
Ebel, Christine
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Coquet, Laurent
论文数: 0引用数: 0
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机构:
Normandie Univ, Inst Res & Innovat Biomed Normandie Rouen, CNRS UMR 6270, Plateforme PISSARO, F-76821 Mont St Aignan, FranceINRA, UMR Biol Fruit & Pathol 1332, F-33882 Villenave Dornon, France
Coquet, Laurent
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Claverol, Stephane
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h-index: 0
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Univ Bordeaux, Funct Genom Ctr Bordeaux, Proteome Platform, F-33076 Bordeaux, FranceINRA, UMR Biol Fruit & Pathol 1332, F-33882 Villenave Dornon, France
Claverol, Stephane
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Vashee, Sanjay
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h-index: 0
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J Craig Venter Inst, Rockville, MD 20850 USAINRA, UMR Biol Fruit & Pathol 1332, F-33882 Villenave Dornon, France
Vashee, Sanjay
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Jores, Joerg
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Int Livestock Res Inst, Nairobi 00100, Kenya
Univ Bern, Inst Vet Bacteriol, CH-3001 Bern, SwitzerlandINRA, UMR Biol Fruit & Pathol 1332, F-33882 Villenave Dornon, France
Jores, Joerg
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Blanchard, Alain
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Sirand-Pugnet, Pascal
论文数: 0引用数: 0
h-index: 0
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INRA, UMR Biol Fruit & Pathol 1332, F-33882 Villenave Dornon, France
Univ Bordeaux, UMR Biol Fruit & Pathol 1332, F-33882 Villenave Dornon, FranceINRA, UMR Biol Fruit & Pathol 1332, F-33882 Villenave Dornon, France
机构:
Univ Missouri, Columbia, MO USAUniv Missouri, Columbia, MO USA
Calcutt, M. J.
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Lysnyansky, I.
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Kimron Vet Inst, Bet Dagan, IsraelUniv Missouri, Columbia, MO USA
Lysnyansky, I.
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Sachse, K.
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机构:
Friedrich Loeffler Inst, Jena, Germany
Friedrich Schiller Univ, Dept RNA Bioinformat & High Throughput Anal, Fac Math & Comp Sci, Jena, GermanyUniv Missouri, Columbia, MO USA
Sachse, K.
;
Fox, L. K.
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Washington State Univ, Pullman, WA 99164 USAUniv Missouri, Columbia, MO USA
Fox, L. K.
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Nicholas, R. A. J.
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机构:
Oaks, Farnham, Surrey, EnglandUniv Missouri, Columbia, MO USA
Nicholas, R. A. J.
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Ayling, R. D.
论文数: 0引用数: 0
h-index: 0
机构:
Anim & Plant Hlth Agcy, Addlestone, Surrey, EnglandUniv Missouri, Columbia, MO USA
机构:
Univ Bordeaux, Inst Europeen Chim & Biol, UMS 3033, F-33607 Pessac, France
SIRIC BRIO, Inst Bergonie, F-33076 Bordeaux, FranceINRA, UMR Biol Fruit & Pathol 1332, F-33882 Villenave Dornon, France
Minder, Laetitia
;
论文数: 引用数:
h-index:
机构:
Di Primo, Carmelo
;
Le Roy, Aline
论文数: 0引用数: 0
h-index: 0
机构:
Univ Grenoble Alpes, Inst Biol Struct, F-38044 Grenoble, France
CNRS, Inst Biol Struct, F-38044 Grenoble, France
CEA, Inst Biol Struct, F-38044 Grenoble, FranceINRA, UMR Biol Fruit & Pathol 1332, F-33882 Villenave Dornon, France
Le Roy, Aline
;
Ebel, Christine
论文数: 0引用数: 0
h-index: 0
机构:
Univ Grenoble Alpes, Inst Biol Struct, F-38044 Grenoble, France
CNRS, Inst Biol Struct, F-38044 Grenoble, France
CEA, Inst Biol Struct, F-38044 Grenoble, FranceINRA, UMR Biol Fruit & Pathol 1332, F-33882 Villenave Dornon, France
Ebel, Christine
;
Coquet, Laurent
论文数: 0引用数: 0
h-index: 0
机构:
Normandie Univ, Inst Res & Innovat Biomed Normandie Rouen, CNRS UMR 6270, Plateforme PISSARO, F-76821 Mont St Aignan, FranceINRA, UMR Biol Fruit & Pathol 1332, F-33882 Villenave Dornon, France
Coquet, Laurent
;
Claverol, Stephane
论文数: 0引用数: 0
h-index: 0
机构:
Univ Bordeaux, Funct Genom Ctr Bordeaux, Proteome Platform, F-33076 Bordeaux, FranceINRA, UMR Biol Fruit & Pathol 1332, F-33882 Villenave Dornon, France
Claverol, Stephane
;
Vashee, Sanjay
论文数: 0引用数: 0
h-index: 0
机构:
J Craig Venter Inst, Rockville, MD 20850 USAINRA, UMR Biol Fruit & Pathol 1332, F-33882 Villenave Dornon, France
Vashee, Sanjay
;
Jores, Joerg
论文数: 0引用数: 0
h-index: 0
机构:
Int Livestock Res Inst, Nairobi 00100, Kenya
Univ Bern, Inst Vet Bacteriol, CH-3001 Bern, SwitzerlandINRA, UMR Biol Fruit & Pathol 1332, F-33882 Villenave Dornon, France
Jores, Joerg
;
论文数: 引用数:
h-index:
机构:
Blanchard, Alain
;
Sirand-Pugnet, Pascal
论文数: 0引用数: 0
h-index: 0
机构:
INRA, UMR Biol Fruit & Pathol 1332, F-33882 Villenave Dornon, France
Univ Bordeaux, UMR Biol Fruit & Pathol 1332, F-33882 Villenave Dornon, FranceINRA, UMR Biol Fruit & Pathol 1332, F-33882 Villenave Dornon, France
机构:
Univ Missouri, Columbia, MO USAUniv Missouri, Columbia, MO USA
Calcutt, M. J.
;
Lysnyansky, I.
论文数: 0引用数: 0
h-index: 0
机构:
Kimron Vet Inst, Bet Dagan, IsraelUniv Missouri, Columbia, MO USA
Lysnyansky, I.
;
Sachse, K.
论文数: 0引用数: 0
h-index: 0
机构:
Friedrich Loeffler Inst, Jena, Germany
Friedrich Schiller Univ, Dept RNA Bioinformat & High Throughput Anal, Fac Math & Comp Sci, Jena, GermanyUniv Missouri, Columbia, MO USA
Sachse, K.
;
Fox, L. K.
论文数: 0引用数: 0
h-index: 0
机构:
Washington State Univ, Pullman, WA 99164 USAUniv Missouri, Columbia, MO USA
Fox, L. K.
;
Nicholas, R. A. J.
论文数: 0引用数: 0
h-index: 0
机构:
Oaks, Farnham, Surrey, EnglandUniv Missouri, Columbia, MO USA
Nicholas, R. A. J.
;
Ayling, R. D.
论文数: 0引用数: 0
h-index: 0
机构:
Anim & Plant Hlth Agcy, Addlestone, Surrey, EnglandUniv Missouri, Columbia, MO USA