MBOVPG45_0375 Encodes an IgG-Binding Protein and MBOVPG45_0376 Encodes an IgG-Cleaving Protein in Mycoplasma bovis

被引:9
作者
Zhao, Haoran [1 ]
Zhang, Yunke [1 ]
Wang, Zhanhui [1 ]
Liu, Mengyao [1 ]
Wang, Pengpeng [1 ]
Wu, Wenxue [1 ]
Peng, Chen [1 ]
机构
[1] China Agr Univ, Coll Vet Med, Key Lab Anim Epidemiol & Zoonosis, Beijing, Peoples R China
关键词
Mycoplasma bovis; MBOVPG45_0375; MBOVPG45_0376; IgG-binding protein; IgG-cleaving protein; membrane protein;
D O I
10.3389/fvets.2021.644224
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
Mycoplasma bovis is a significant bacterial pathogen which is able to persist in cattle and cause chronic diseases. This phenomenon may relate to M. bovis evading the immune system of the host. Immunoglobulin-binding proteins are widely distributed in a variety of pathogenic bacteria, including some Mycoplasma species. These proteins are considered to help the bacteria evade the immune response of the host. Here we found M. bovis strain PG45 can bind to IgG from several animals. MBOVPG45_0375 encodes a putative membrane protein, has strong amino acid sequence similarity with Immunoglobulin G-binding protein in Mycoplasma mycoides subsp. capri. Hence, we constructed recombinant MBOVPG45_0375 (r0375) in the Escherichia coli expression system and demonstrated that r0375 can bind to IgG non-immunologically rather than specific binding similar to interaction of antigen and antibody. Moreover, r0375 can bind to the Fab fragment of IgG. Also, the binding of r0375 and IgG inhibits the formation of antigen-antibody union. Furthermore, MBOVPG45_0376 encodes an IgG-cleaving protein of M. bovis strain PG45. Nevertheless, r0375 binding to IgG is required for the cleavage activity of recombinant 0376 (r0376). The activity of r0376 is also affected by incubation time and temperature. In addition, we found both MBOVPG45_0375 and MBOVPG45_0376 are membrane proteins of M. bovis strain PG45. These results about MBOVPG45_0375 as an IgG-binding protein and MBOVPG45_0376 as an IgG-cleaving protein offer a new insight into the interaction between M. bovis and its host.
引用
收藏
页数:11
相关论文
共 36 条
[1]   IDENTIFICATION OF A MYCOPLASMAL PROTEIN WHICH BINDS IMMUNOGLOBULINS NONIMMUNOLOGICALLY [J].
ALEXANDER, AG ;
LOWES, HR ;
KENNY, GE .
INFECTION AND IMMUNITY, 1991, 59 (06) :2147-2151
[2]   MIB-MIP is a mycoplasma system that captures and cleaves immunoglobulin G [J].
Arfi, Yonathan ;
Minder, Laetitia ;
Di Primo, Carmelo ;
Le Roy, Aline ;
Ebel, Christine ;
Coquet, Laurent ;
Claverol, Stephane ;
Vashee, Sanjay ;
Jores, Joerg ;
Blanchard, Alain ;
Sirand-Pugnet, Pascal .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2016, 113 (19) :5406-5411
[3]   Mycoplasma bovis isolated from brain tissue of calves [J].
Ayling, R ;
Nicholas, R ;
Hogg, R ;
Wessels, J ;
Scholes, S ;
Byrne, W ;
Hill, M ;
Moriarty, J ;
O'Brien, T .
VETERINARY RECORD, 2005, 156 (12) :391-392
[4]   A FAMILY OF PHASE-VARIANT AND SIZE-VARIANT MEMBRANE-SURFACE LIPOPROTEIN ANTIGENS (VSPS) OF MYCOPLASMA-BOVIS [J].
BEHRENS, A ;
HELLER, M ;
KIRCHHOFF, H ;
YOGEV, D ;
ROSENGARTEN, R .
INFECTION AND IMMUNITY, 1994, 62 (11) :5075-5084
[5]  
Braun V, 2019, SUBCELL BIOCHEM, V92, P39, DOI 10.1007/978-3-030-18768-2_3
[6]   Gap analysis of Mycoplasma bovis disease, diagnosis and control: An aid to identify future development requirements [J].
Calcutt, M. J. ;
Lysnyansky, I. ;
Sachse, K. ;
Fox, L. K. ;
Nicholas, R. A. J. ;
Ayling, R. D. .
TRANSBOUNDARY AND EMERGING DISEASES, 2018, 65 :91-109
[7]   Mycoplasmas and their host: emerging and re-emerging minimal pathogens [J].
Citti, Christine ;
Blanchard, Alain .
TRENDS IN MICROBIOLOGY, 2013, 21 (04) :196-203
[8]   Genetic dissection of the Streptococcus pyogenes M1 protein:: regions involved in fibronectin binding and intracellular invasion [J].
Cue, D ;
Lam, H ;
Cleary, PP .
MICROBIAL PATHOGENESIS, 2001, 31 (05) :231-242
[9]   Development of a direct competitive ELISA for the detection of Mycoplasma bovis infection based on a monoclonal antibody of P48 protein [J].
Fu, Ping ;
Sun, Zhenhong ;
Zhang, Yuewei ;
Yu, Ziqiang ;
Zhang, Haiyan ;
Su, Dan ;
Jiang, Fei ;
Wu, Wenxue .
BMC VETERINARY RESEARCH, 2014, 10
[10]   Naturally occurring Mycoplasma bovis-associated pneumonia and polyarthritis in feedlot beef calves [J].
Gagea, MI ;
Bateman, KG ;
Shanahan, RA ;
van Dreumel, T ;
McEwen, BJ ;
Carman, S ;
Archambault, M ;
Caswell, JL .
JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION, 2006, 18 (01) :29-40