DNA Methylation in Human Breast Cancer Cell Lines Adapted to High Nitric Oxide

被引：5
作者：
Demircan, Berna [1 ]
Yucel, Burcu [1 ]
Radosevich, James A. [2 ]
机构：
[1] Istanbul Medeniyet Univ, Med Sch, Dept Med Biol, TR-34000 Istanbul, Turkey
[2] Univ Illinois, Coll Dent, Oral Med & Diagnost Sci, Chicago, IL USA
来源：
IN VIVO | 2020年 / 34卷 / 01期
关键词：
DNA methylation; epigenetics; breast cancer; nitric oxide; BRCA1 PROMOTER METHYLATION; TUMOR-SUPPRESSOR GENES; EPIGENETIC MECHANISMS; BT-20; BT-20-HNO; ADAPTATION; EXPRESSION; RASSF1A; INTERLEUKIN-1-BETA; ASSOCIATION; PROGRESSION;
D O I：
10.21873/invivo.11758
中图分类号：
R-3 [医学研究方法]; R3 [基础医学];
学科分类号：
1001 ;
摘要：
Background: Nitric oxide (NO) exposure has been suggested to cause alterations in DNA methylation in breast cancer. We investigated the effect of NO on DNA methylation of promoters in cell lines of breast cancer. Material and Methods: The methylation status of the promoters of breast cancer 1 (BRCA1), deleted in colon cancer (DCC), Ras-association domain family 1A (RASSF1A), O-6-methylguanineDNA methyltransferase (MGMT), and secreted frizzled related protein 1 (SFRP1) were analyzed in the parental and high nitric oxide-adapted cell lines of breast cancer using Illumina MiSequencing. Results: Methylation of RASSF1A promoter in BT-20-HNO (74.7%) was significantly higher than that in BT-20 cells (72%) (p<0.05), whereas in MCF-7-HNO cells, methylation of MGMT promoter was found to have significantly decreased as compared to its parental cell line (45.1% versus 50.1%; p<0.0001). Promoter methylation of SFRP and DCC was elevated in T-47D-HNO relative to its parent cell line (p<0.05). Conclusion: Similarly to the double-edged effects of NO on tumorigenesis, its epigenetic effects through DNA methylation are diverse and contradictory in breast cancer.
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页码：169 / 176
页数：8
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