Characterization of cold-induced remodelling reveals depot-specific differences across and within brown and white adipose tissues in mice

被引:52
作者
Jia, R. [1 ,2 ,3 ]
Luo, X. -Q. [4 ]
Wang, G. [5 ]
Lin, C. -X. [1 ,2 ]
Qiao, H. [1 ,2 ]
Wang, N. [1 ,2 ]
Yao, T. [1 ,2 ]
Barclay, J. L. [6 ,7 ]
Whitehead, J. P. [6 ,7 ]
Luo, X. [1 ,2 ]
Yan, J. -Q. [1 ,2 ]
机构
[1] Xi An Jiao Tong Univ, Hlth Sci Ctr, Sch Basic Med Sci, Dept Physiol & Pathophysiol, Xian, Peoples R China
[2] Xi An Jiao Tong Univ, Key Lab Environm & Genes Related Dis, Minist Educ China, Xian, Peoples R China
[3] Xi An Jiao Tong Univ, Stomatol Hosp, Dept Prosthodont, Coll Stomatol, Xian, Peoples R China
[4] Xi An Jiao Tong Univ, Hlth Sci Ctr, Sch Publ Hlth, Dept Med, Xian, Peoples R China
[5] Boston Univ, Dept Biol, Boston, MA 02215 USA
[6] Univ Queensland, Mater Res Inst, Brisbane, Qld, Australia
[7] Translat Res Inst, Brisbane, Qld, Australia
基金
英国医学研究理事会; 中国博士后科学基金; 中国国家自然科学基金;
关键词
beige cells; brown adipose tissue; cold exposure; time course; uncoupling protein 1; GENE-EXPRESSION; ADULT HUMANS; NONSHIVERING THERMOGENESIS; ENERGY-EXPENDITURE; C57BL/6J MICE; FAT DEPOTS; ADIPOCYTES; OBESITY; MOUSE; ACTIVATION;
D O I
10.1111/apha.12688
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
AimBrown and beige adipose tissues dissipate energy in the form of heat via mitochondrial uncoupling protein 1, defending against hypothermia and potentially obesity. The latter has prompted renewed interest in understanding the processes involved in browning to realize the potential therapeutic benefits. To characterize the temporal profile of cold-induced changes and browning of brown and white adipose tissues in mice. MethodsMale C57BL/6J mice were singly housed in conventional cages under cold exposure (4 degrees C) for 1, 2, 3, 4, 5 and 7days. Food intake and body weight were measured daily. Interscapular brown adipose tissue (iBAT), inguinal subcutaneous (sWAT) and epididymal white adipose tissue (eWAT) were harvested for histological, immunohistochemical, gene and protein expression analysis. ResultsUpon cold exposure, food intake increased, whilst body weight and adipocyte size were found to be transiently reduced. iBAT mass was found to be increased, whilst sWAT and eWAT were found to be transiently decreased. A combination of morphological, genetic (Ucp-1, Pgc-1 and Elov13) and biochemical (UCP-1, PPAR and aP2) analyses demonstrated the depot-specific remodelling in response to cold exposure. ConclusionOur results demonstrate the differential responses to cold-induced changes across discrete BAT and WAT depots and support the notion that the effects of short-term cold exposure are achieved by expansion, activation and increasing thermogenic capacity of iBAT, as well as browning of sWAT and, to a lesser extent, eWAT.
引用
收藏
页码:311 / 324
页数:14
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