Sphingomonas panacisoli sp. nov., bacterium isolated from soil in South Korea

A newly isolated bacterial strain designated as HKS19 was isolated from a ginseng cultivation soil sample collected in South Korea. Cells of the strain HKS19 were Gram-stain-negative, rod, oval-shaped and they formed yellow colonies when grown on R2A agar at 30 degrees C. HKS19 showed the highest 16S rRNA gene sequence similarity (98.6%) with Sphingomonas asaccharolytica NBRC 15499(T). Its growth was observed at 10-37 degrees C (optimum 30 degrees C), pH 6-9 (optimum pH 7), and in the presence of 0-1% NaCl (optimum 0%). The genome size of HKS19 was 3.4 Mb and the G+C content was 65.1 mol%. The main polar lipid of strain HKS19 was diphosphatidylglycerol (DPG), the predominant respiratory quinone was Q-10 and the major fatty acids were a summed feature 8 (C-18:1 omega 6c / C-18:1 omega 7c) and C-16:0. Based on the phylogenetic, genotypic, phenotypic and chemotaxonomic analysis, strain HKS19 represents a newly isolated species of the genus Sphingomonas, for which the name Sphingomonas panacisoli is proposed. The type strain is HKS19(T) (=KACC 18881(T)=LMG 29564(T)).