Strengths and weaknesses of methods for identifying monoclonal free light chains of Ig: examples from two cases with renal disease

被引：3

作者：

Levinson, Stanley S. ^{[1
,2
,3
]}

机构：

[1] Robley Rex VA Med Ctr, Pathol & Lab Med, 800 Zorn Ave, Louisville, KY 40206 USA

[2] Univ Louisville, Dept Vet Affairs Med Ctr, Louisville, KY 40292 USA

[3] Univ Louisville, Dept Med, Endocrinol Sect, Louisville, KY 40292 USA

来源：

CLINICAL CHEMISTRY AND LABORATORY MEDICINE | 2016年 / 54卷 / 06期

关键词：

free light chains; immunofixation electrophoresis; serum; urine; SERUM; OVERESTIMATION; IMMUNOFIXATION; CRITERIA; URINE; ASSAY;

D O I：

10.1515/cclm-2015-0482

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

Background: Serum free light chain (FLC) analysis with ratio and urine immunofixation electrophoresis (IFE) are both available for routine use in helping to detect plasma cell dyscrasia and related diseases. Cases: Case reports showing one serum positive for serum FLC but that showed a hook effect and overestimated the amount of monoclonal FLC while urine IFE was negative for Bence Jones protein, and a second serum that showed elevated FLC kappa and lambda but a normal kappa/lambda ratio, while urine IFE was positive for Bence Jones protein. Conclusions: These two techniques complement one another. Neither of the techniques is truly quantitative, and both exhibit methodological defects.

引用

页码：1039 / 1043

页数：5

共 16 条

[1] Overestimation of Serum κ Free Light Chain Concentration by Immunonephelometry [J].

Angelino, Corrie M. de Kat ;

Raymakers, Reinier ;

Teunesen, Maria A. ;

Jacobs, Joannes F. M. ;

Klasen, Ina S. .

CLINICAL CHEMISTRY, 2010, 56 (07) :1188-1190

[2] Detection of serum free light chains: the problem with antigen excess [J].

Bosmann, Markus ;

Koessler, Juergen ;

Stolz, Herbert ;

Walter, Ulrich ;

Knop, Stefan ;

Steigerwald, Udo .

CLINICAL CHEMISTRY AND LABORATORY MEDICINE, 2010, 48 (10) :1419-1422

[3]

Bradwell AR, 2001, CLIN CHEM, V47, P673

[4] Analytical performance of the serum free light chain assay [J].

Briand, Pierre-Yves ;

Decaux, Olivier ;

Caillon, Helene ;

Grosbois, Bernard ;

Le Treut, Andre ;

Guenet, Lucienne .

CLINICAL CHEMISTRY AND LABORATORY MEDICINE, 2010, 48 (01) :73-79

[5] International Myeloma Working Group guidelines for serum-free light chain analysis in multiple myeloma and related disorders [J].

Dispenzieri, A. ;

Kyle, R. ;

Merlini, G. ;

Miguel, J. S. ;

Ludwig, H. ;

Hajek, R. ;

Palumbo, A. ;

Jagannath, S. ;

Blade, J. ;

Lonial, S. ;

Dimopoulos, M. ;

Comenzo, R. ;

Einsele, H. ;

Barlogie, B. ;

Anderson, K. ;

Gertz, M. ;

Harousseau, J. L. ;

Attal, M. ;

Tosi, P. ;

Sonneveld, P. ;

Boccadoro, M. ;

Morgan, G. ;

Richardson, P. ;

Sezer, O. ;

Mateos, M. V. ;

Cavo, M. ;

Joshua, D. ;

Turesson, I. ;

Chen, W. ;

Shimizu, K. ;

Powles, R. ;

Rajkumar, S. V. ;

Durie, B. G. M. .

LEUKEMIA, 2009, 23 (02) :215-224

[6] International uniform response criteria for multiple myeloma [J].

Durie, B. G. M. ;

Harousseau, J-L ;

Miguel, J. S. ;

Blade, J. ;

Barlogie, B. ;

Anderson, K. ;

Gertz, M. ;

Dimopoulos, M. ;

Westin, J. ;

Sonneveld, P. ;

Ludwig, H. ;

Gahrton, G. ;

Beksac, M. ;

Crowley, J. ;

Belch, A. ;

Boccadaro, M. ;

Turesson, I. ;

Joshua, D. ;

Vesole, D. ;

Kyle, R. ;

Alexanian, R. ;

Tricot, G. ;

Attal, M. ;

Merlini, G. ;

Powles, R. ;

Richardson, P. ;

Shimizu, K. ;

Tosi, P. ;

Morgan, G. ;

Rajkumar, S. V. .

LEUKEMIA, 2006, 20 (09) :1467-1473

[7] Quantitative Assessment of Serum and Urinary Polyclonal Free Light Chains in Patients with Chronic Kidney Disease [J].

Hutchison, Colin A. ;

Harding, Stephen ;

Hewins, Pete ;

Mead, Graham P. ;

Townsend, John ;

Radwell, Arthur R. B. ;

Cockwell, Paul .

CLINICAL JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY, 2008, 3 (06) :1684-1690

[8] Elimination of the need for urine studies in the screening algorithm for monoclonal gammopathies by using serum immunofixation and free light chain assays [J].

Katzmann, Jerry A. ;

Dispenzieri, Angela ;

Kyle, Robert A. ;

Snyder, Melissa R. ;

Plevak, Matthew F. ;

Larson, Dirk R. ;

Abraham, Roshini S. ;

Lust, John A. ;

Melton, L. Joseph, III ;

Rajkumar, S. Vincent .

MAYO CLINIC PROCEEDINGS, 2006, 81 (12) :1575-1578

[9]

Keren DF, 1999, ARCH PATHOL LAB MED, V123, P106

[10]

LEVINSON SS, 1992, ANN CLIN LAB SCI, V22, P100

← 1 2 →