Circular RNA UVRAG Mediated by Alternative Splicing Factor NOVA1 Regulates Adhesion and Migration of Vascular Smooth Muscle Cells

被引：12

作者：

Liu, Ze ^{[1
]}

Lou, Yue ^{[1
]}

Cui, Jia-Chen ^{[1
]}

Chen, Yi ^{[1
]}

Liu, Ji-Ting ^{[1
]}

Yuan, Ying ^{[2
]}

Han, Yue ^{[1
]}

Huo, Yun-Long ^{[1
]}

Qi, Ying-Xin ^{[1
,3
,4
]}

Jiang, Zong-Lai ^{[1
]}

Yao, Qing-Ping ^{[1
]}

机构：

[1] Shanghai Jiao Tong Univ, Sch Life Sci & Biotechnol, Inst Mechanobiol & Med Engn, Shanghai 200240, Peoples R China

[2] Shanghai Jiao Tong Univ, Shanghai Gen Hosp, Sch Med, Dept Ophthalmol, Shanghai 200080, Peoples R China

[3] Beihang Univ, Sch Biol Sci & Med Engn, Key Lab Biomech & Mechanobiol, Minist Educ, Beijing 100083, Peoples R China

[4] Beihang Univ, Beijing Adv Innovat Ctr Biomed Engn, Beijing 100083, Peoples R China

来源：

GENES | 2021年 / 12卷 / 03期

基金：

中国国家自然科学基金;

关键词：

vascular smooth muscle cells; circular RNAs; vein graft; adhesion; migration; alternative splicing; NOVA1; GROWTH; IDENTIFICATION; SUPPRESSION; HYPERPLASIA; PROTEIN; ARTERY;

D O I：

10.3390/genes12030418

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The movement of abnormal vascular smooth muscle cells (VSMCs) contributes to intimal hyperplasia in vein graft disease. Circular RNAs (circRNAs) are single stranded RNAs with 3' and 5' ends covalently joined together. They have been shown to regulate cell function in many diseases. NOVA1 is considered to be a brain-specific splicing factor that plays an important role in the nervous system and cancer. The role of NOVA1 in VSMCs remains unclear. In the present study, transcriptome sequencing was used to identify differentially expressed circRNAs in the rat vein graft model. A novel circRNA, circUVRAG, was decreased in the grafted vein and stably located in the cytoplasm. Knockdown of circUVRAG suppressed VSMC adhesion and migration. In addition, we demonstrated that the alternative splicing factor NOVA1 co-located with UVRAG pre-mRNA in the nucleus and modulated the production of circUVRAG. These new discoveries may serve as a potential means to treat intimal hyperplasia after vein grafts.

引用

页数：13

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