Radical-induced oxidation of metformin

被引：48
作者：
Khouri, H
Collin, F
Bonnefont-Rousselot, D
Legrand, A
Jore, D
Gardès-Albert, M
机构：
[1] Univ Paris 05, CNRS, UMR 8601, Chim Phys Lab, F-75270 Paris 06, France
[2] Fac Pharm, Lab Biochim Metab & Clin, Paris 5, France
[3] Hop La Pitie Salpetriere, APHP, Lab Biochim B, Paris, France
来源：
EUROPEAN JOURNAL OF BIOCHEMISTRY | 2004年 / 271卷 / 23-24期
关键词：
metformin; hydroxyl radical; antioxidant; radiolysis;
D O I：
10.1111/j.1432-1033.2004.04438.x
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Metformin (1,1-dimethylbiguanide) is an antihyperglycaemic drug used to normalize glucose concentrations in type 2 diabetes. Furthermore, antioxidant benefits have been reported in diabetic patients treated with metformin. This work was aimed at studying the scavenging capacity of this drug against reactive oxygen species (ROS) like (OH)-O-. and O-2(-.) -free radicals. ROS were produced by gamma radiolysis of water. The irradiated solutions of metformin were analyzed by UV/visible absorption spectrophotometry. It has been shown that hydroxyl free radicals react with metformin in a concentration-dependent way. The maximum scavenging activity was obtained for concentrations of metformin greater than or equal to 200 mumol.L-1, under our experimental conditions. An estimated value of 10(7) L.mol(-1).s(-1) has been determined for the second order rate constant k((OH)-O-. + metformin). Superoxide free radicals and hydrogen peroxide do not initiate any oxidation on metformin in our in vitro experiments.
引用
收藏
页码：4745 / 4752
页数：8
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