Proteome-wide Lysine Glutarylation Profiling of the Mycobacterium tuberculosis H37Rv

被引:31
作者
Xie, Longxiang [1 ]
Wang, Guirong [2 ]
Yu, Zhaoxiao [1 ]
Zhou, Mingliang [1 ]
Li, Qiming [1 ]
Huang, Hairong [2 ]
Xie, Jianping [1 ]
机构
[1] Southwest Univ, State Key Lab Breeding Base Ecoenvironm & Bioreso, Key Lab Ecoenvironm Three Gorges Reservoir Reg, Minist Educ,Sch Life Sci, Chongqing 400715, Peoples R China
[2] Capital Med Univ, Natl Clin Lab TB, Beijing Key Lab Drug Resistant TB Res, Beijing Chest Hosp,Beijing TB & Thorac Tumor Inst, Beijing 101149, Peoples R China
关键词
posttranslational modification; glutarylated proteins; global glutarylome; E; coli; stress reaction; VIBRIO-PARAHEMOLYTICUS; SYSTEMATIC ANALYSIS; ANALYSIS REVEALS; ACETYLATION; SUCCINYLATION; ACETYLOME; ACYLTRANSFERASE; BIOSYNTHESIS; INVOLVEMENT; RV1347C;
D O I
10.1021/acs.jproteome.5b00917
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Lysine glutarylation, a new protein posttranslational modification (PTM), was recently identified and characterized in both prokaryotic and eukaryotic cells. To explore the distribution of lysine glutarylation in Mycobacterium tuberculsosis, by using a comprehensive method combining the immune affinity peptide enrichment by the glutaryl-lysine antibody with LC-MS, we finally identified 41 glutarylation sites in 24 glutarylated proteins from M. tuberculosis. These glutarylated proteins are involved in various cellular functions such as translation and metabolism and exhibit diverse subcellular localizations. Three common glutarylated proteins including SOS ribosomal protein L7/L12, elongation factor Tu, and dihydrolipoamide succinyltransferase are shared between Escherichia coli and M. tuberculosis. Moreover, comparison with other PTMs characterized in M. tuberculosis, 15 glutarylated proteins, are found to be both acetylated and succinylated. Notably, several stress-response-associated proteins including HspX are glutarylated. Our data provide the first analysis of M. tuberculosis lysine glutarylated proteins. Further studies on the role of the glutarylated proteins will unveil the molecular mechanisms of glutarylation underlying M. tuberculosis physiology and pathogenesis.
引用
收藏
页码:1379 / 1385
页数:7
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