Cloning and sequence analysis of the cDNA encoding the elongation factor-1β of Dictyostelium discoideum

被引：10

作者：

Chae, SC ^{[1
]}

Maeda, Y ^{[1
]}

机构：

[1] Tohoku Univ, Grad Sch Sci, Inst Biol, Aoba Ku, Sendai, Miyagi 98077, Japan

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY | 1998年 / 1383卷 / 01期

关键词：

elongation factor-1 beta (DdEF-1 beta); protein synthesis; differential display (DD); cDNA; (Dictyostelium);

D O I：

10.1016/S0167-4838(97)00220-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A cDNA encoding elongation factor-1 beta (DdEF-1 beta) for protein synthesis was isolated from Dictyostlium discoideum cells by the differential display (DD) method. The DdEF-1 beta cDNA sequence with a length of 733 bp encodes a protein (M-r 24.1 kDa) consisting of 214 amino acids. Comparison of the predicted amino acid sequence revealed relatively high homology to that of DdEF-1 alpha from silkworm (59%), human (57%) and Xenopus (55%). Northern analysis showed that the DdEF-1 beta mRNA is maximally expressed during the vegetative growth phase, followed by marked decrease in response to cell differentiation induced by starvation. (C) 1998 Elsevier Science B.V.

引用

页码：1 / 3

页数：3

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