Microbial lipids from industrial wastes using xylose-utilizing Ashbya gossypii strains

被引：19

作者：

Diaz-Fernandez, David ^{[1
]}

Aguiar, Tatiana Q. ^{[2
]}

Isabel Martin, Victoria ^{[1
]}

Romani, Aloia ^{[2
]}

Silva, Rui ^{[2
]}

Domingues, Lucilia ^{[2
]}

Luis Revuelta, Jose ^{[1
]}

Jimenez, Alberto ^{[1
]}

机构：

[1] Univ Salamanca, Dept Microbiol & Genet, Metab Engn Grp, Campus Miguel de Unamuno, E-37007 Salamanca, Spain

[2] Univ Minho, CEB Ctr Biol Engn, P-4710057 Braga, Portugal

来源：

BIORESOURCE TECHNOLOGY | 2019年 / 293卷

关键词：

Ashbya gossypii; Microbial lipids; Regulation; Lignocellulosic hydrolysates; Xylose; Molasses; Glycerol; RIBOFLAVIN PRODUCTION; GLYCEROL; FERMENTATION; HYDROLYSATE; MOLASSES; PATHWAY; SUGARS;

D O I：

10.1016/j.biortech.2019.122054

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

This work presents the exploitation of waste industrial by-products as raw materials for the production of microbial lipids in engineered strains of the filamentous fungus Ashbya gossypii. A lipogenic xylose-utilizing strain was used to apply a metabolic engineering approach aiming at relieving regulatory mechanisms to further increase the biosynthesis of lipids. Three genomic manipulations were applied: the overexpression of a feedback resistant form of the acetyl-CoA carboxylase enzyme; the expression of a truncated form of Mga2, a regulator of the main Delta 9 desaturase gene; and the overexpression of an additional copy of DGA1 that codes for diacylglycerol acyltransferase. The performance of the engineered strain was evaluated in culture media containing mixed formulations of corn-cob hydrolysates, sugarcane molasses or crude glycerol. Our results demonstrate the efficiency of the engineered strains, which were able to accumulate about 40% of cell dry weight (CDW) in lipid content using organic industrial wastes as feedstocks.

引用

页数：8

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