A Dual-Modal Molecular Probe for Near-Infrared Fluorescence and Photoacoustic Imaging of Peroxynitrite

被引：168

作者：

Zhang, Jianjian ^{[1
,2
]}

Zhen, Xu ^{[2
]}

Zeng, Jianfeng ^{[3
]}

Pu, Kanyi ^{[2
]}

机构：

[1] Northwest Univ, Coll Chem & Mat Sci, Key Lab Synthet & Nat Funct Mol Chem, Minist Educ, Xian 710127, Shaanxi, Peoples R China

[2] Nanyang Technol Univ, Sch Chem & Biomed Engn, Singapore 637457, Singapore

[3] Soochow Univ, State Key Lab Radiat Med & Protect, Suzhou 215123, Peoples R China

来源：

ANALYTICAL CHEMISTRY | 2018年 / 90卷 / 15期

关键词：

SEMICONDUCTING POLYMER NANOPARTICLES; SELECTIVE DETECTION; REACTIVE OXYGEN; IN-VITRO; PROTEIN; CANCER; THERANOSTICS; TOMOGRAPHY; NANOSHEETS; OLIGOMER;

D O I：

10.1021/acs.analchem.8b01879

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Peroxynitrite (ONOO-), a reactive and short-lived biological oxidant, is closely related with many pathological conditions such as cancer. However, real-time in vivo imaging of ONOO- in tumors remains to be challenging. Herein, we develop a near-infrared fluorescence (NIRF) and photoacoustic dual-modal molecular probe (CySO3CF3) composed of a water-soluble hemicyanine dye caged with a trifluoromethyl ketone moiety for in vivo imaging of ONOO-. The trifluoromethyl ketone moiety can undergo a series of ONOO--induced cascade oxidation-elimination reactions, leading to sensitive and specific fluorescence and photoacoustic turn-on responses toward ONOO-; whereas, a zwitterionic structure of the hemicyanine component ensures good water-solubility. Thus, CySO3CF3 not only specifically detects ONOO- in solution and cells with the limit of detection down to 53 nM but also allows for NIRF and photoacoustic dual-modal imaging of ONOO- in the tumors of living mice.

引用

页码：9301 / 9307

页数：7

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[1] Peroxynitrite reactivity with amino acids and proteins [J].