Molecular characterization of Mycobacterium tuberculosis through MPT64 gene polymorphism using next-generation sequencing technology

被引：1

作者：

Zamir, Hina ^{[1
]}

Ahmad, Bashir ^{[1
]}

Ali, Sajid ^{[2
]}

Khan, Saad A. ^{[3
]}

Sarwar, Raheela ^{[1
]}

Khan, Alamzeb ^{[4
]}

Ali, Johar ^{[5
]}

Khan, Abid A. ^{[1
,5
,6
]}

机构：

[1] Univ Peshawar, Ctr Biotechnol & Microbiol, Peshawar 25120, KP, Pakistan

[2] Prov TB Reference Lab, Peshawar 25120, KP, Pakistan

[3] Kabir Med Coll, Dept Med, Peshawar 25120, Kp, Pakistan

[4] Yale Univ, Yale Sch Med, Dept Pediat, New Haven, CT 06511 USA

[5] Rehman Med Inst, Ctr Genom Sci, Peshawar 25120, KP, Pakistan

[6] Univ Lakki Marwat, Dept Chem Sci, Lakki Marwat 25120, KP, Pakistan

来源：

FUTURE MICROBIOLOGY | 2022年 / 17卷 / 10期

关键词：

Biomarker; diagnosis; M; tuberculosis; MPT64; gene; mutation; NGS; polymorphism; TBC IDENTIFICATION TEST; CULTURE CONFIRMATION; RAPID IDENTIFICATION; 63-BP DELETION; ANTIGEN MPT64; CAPILIA TB; COMPLEX; PROTEIN; ASSAY; DIAGNOSIS;

D O I：

10.2217/fmb-2020-0231

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Aim: To characterize and analyze polymorphism of the MPT64 gene and evaluate AgMPT64-based immunochromatographic assay (ICA) specificity associated with polymorphism. Materials & methods: A total of 1449 suspected samples were tested for tuberculosis (TB), and the MPT64/rv1980c gene was sequenced using next-generation sequencing for polymorphism analysis. Results: Of the TB-positive individuals, 200 (13.80%), 186 (12.84%) and 129 (8.90%) were positive using the liquid culture, GeneXpert and fluorescence microscopy assays, respectively. Liquid culture medium-based samples were confirmed using ICA, in which 193 (96.5%) were positive while seven (3.5%) were negative. Out of 14 sequenced samples, seven were positive and seven negative; 13 were identical to the reference and just one (ICA positive) showed a C477A point mutation (F159L). Conclusion: The results indicate that AgMPT64 can be considered as a potent vaccine candidate.

引用

页码：763 / 772

页数：10

共 51 条

[1]

Afridi OK., 2022, CURR MICROBIOL, V79, P1

[2] Next-Generation Sequencing Based Gut Resistome Profiling of Broiler Chickens Infected with Multidrug-Resistant Escherichia coli [J].

Afridi, Ome Kalsoom ;

Ali, Johar ;

Chang, Jeong Ho .

ANIMALS, 2020, 10 (12) :1-11

[3] KLF17 attenuates estrogen receptor α-mediated signaling by impeding ERα function on chromatin and determines response to endocrine therapy [J].

Ali, Amjad ;

Ielciu, Irina ;

Alkreathy, Huda Mohammad ;

Khan, Abid Ali .

BIOCHIMICA ET BIOPHYSICA ACTA-GENE REGULATORY MECHANISMS, 2016, 1859 (07) :883-895

[4]

Ali J, 2021, CURR TRENDS OMICS, V1, P01

[5]

[Anonymous], 2019, SD BIOLINE TB AG MPT

[6]

[Anonymous], 2018, Tuberculosis

[7]

Baratloo A, 2015, EMERGENCY, V3, P48

[8] Evaluation of the BD MGIT™ TBc identification test for rapid identification of Mycobacterium tuberculosis Complex from positive BACTEC MGIT 960 cultures in a routine laboratory work [J].

Barouni, A. S. ;

Alnajh, Z. B. ;

Aboguttaia, N. B. ;

Alamri, W. M. .

AFRICAN JOURNAL OF MICROBIOLOGY RESEARCH, 2012, 6 (05) :1065-1068

[9] False-negative BD MGIT™ TBc Identification Test results in routine tuberculosis diagnosis: a New Zealand perspective [J].

Basu, I. ;

Bower, J. E. ;

Henderson, G. K. ;

Lowe, O. ;

Newton, S. ;

Vaughan, R. ;

Roberts, S. A. .

INTERNATIONAL JOURNAL OF TUBERCULOSIS AND LUNG DISEASE, 2015, 19 (09) :1073-1075

[10] Trimmomatic: a flexible trimmer for Illumina sequence data [J].

Bolger, Anthony M. ;

Lohse, Marc ;

Usadel, Bjoern .

BIOINFORMATICS, 2014, 30 (15) :2114-2120

← 1 2 3 4 5 6 →