Effects of different follicle-stimulating hormone (FSH)/luteinizing hormone (LH) ratios on follicular development and steroidogenesis in mice

被引：0
作者：
Li, Ya-Fang [1 ]
Guo, Lei [2 ]
Quan, Song [3 ]
Ou, Xiang-Hong [2 ]
机构：
[1] Air Force Hosp Southern Theater, Dept Obstet & Gynecol, Guangzhou 510602, Guangdong, Peoples R China
[2] 2nd Hosp Guangdong Prov, Reprod Med Ctr, 466 Newport Rd, Guangzhou 510317, Guangdong, Peoples R China
[3] Southern Med Univ, Nanfang Hosp, Dept Obstet & Gynecol, Reprod Med Ctr, 1838 Guangzhou North Rd, Guangzhou 510515, Guangdong, Peoples R China
来源：
INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE | 2019年 / 12卷 / 07期
关键词：
FSH/LH ratio; follicular development; steroidogenesis; gene expression; CONTROLLED OVARIAN STIMULATION; ACUTE REGULATORY PROTEIN; RECOMBINANT HUMAN LH; LUTEINIZING-HORMONE; GRANULOSA-CELLS; CHORIONIC-GONADOTROPIN; SIGNALING PATHWAYS; GENE-EXPRESSION; PREGNANCY RATES; FSH;
D O I：
暂无
中图分类号：
R-3 [医学研究方法]; R3 [基础医学];
学科分类号：
1001 ;
摘要：
Objective: The aim of the current study was to investigate the effects of different follicle-stimulating hormone (FSH)/luteinizing hormone (LH) ratios on follicular growth and hormone secretion. Materials and methods: Immature mice were treated with pregnant mare serum gonadotrophin (PMSG) + human chorionic gonadotropin (hCG), as well as different FSH/LH ratios and hCG. Morphological changes and expression levels of fertility-related genes in the ovaries were examined. Results: Treatment with hCG (8 hours) significantly promoted follicular development in the 1:1 FSH/LH (F/L) group. However, atretic follicles and luteinization were observed in the 1:2 group (F/2L). Moreover, mRNA levels of steroidogenesis-related (LHCGR, STARD1, SULT1E1, and CYP11A1), oocyte maturation- related (AREG, BTC, and EREG), and cumulus expansion-related (HAS2, PTGS2, TNFAIP6, and PTX3) genes were significantly upregulated in PMSG and F/L groups at 4 hours and 8 hours after hCG treatment. Expression levels, however, were obviously inhibited in the F/2L group. Conclusion: An appropriate ratio (1:1) of FSH/LH can effectively induce steroidogenesis and follicular development.
引用
收藏
页码：8415 / 8426
页数：12
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