The challenges of the analysis of basic compounds by high performance liquid chromatography: Some possible approaches for improved separations

被引：208
作者：
McCalley, David V. [1 ]
机构：
[1] Univ W England, Ctr Biomed Res, Bristol BS16 1QY, Avon, England
来源：
JOURNAL OF CHROMATOGRAPHY A | 2010年 / 1217卷 / 06期
关键词：
HPLC; Basic compounds; Stationary phases; Reversed-phase; HILIC; REVERSED-PHASE COLUMNS; HYDROPHILIC-INTERACTION CHROMATOGRAPHY; POLYBUTADIENE-COATED ZIRCONIA; HYDROPHOBIC-SUBTRACTION MODEL; PRINCIPAL COMPONENT ANALYSIS; BUFFERED MOBILE PHASES; MASS-TRANSFER KINETICS; C18 STATIONARY PHASES; ADSORPTION MECHANISM; IONIZABLE COMPOUNDS;
D O I：
10.1016/j.chroma.2009.11.068
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
This review considers some of the difficulties encountered with the analysis of ionised bases using reversed-phase chromatography, such as detrimental interaction with column silanol groups, and overloading which both lead to poor peak shapes. Methods of overcoming these problems in reversed-phase (RP) separations, by judicious selection of the column and mobile phase conditions, are discussed Hydrophilic interaction chromatography is considered as an alternative method for the separation of some basic compounds. (C) 2009 Elsevier B.V. All rights reserved.
引用
收藏
页码：858 / 880
页数：23
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