The challenges of the analysis of basic compounds by high performance liquid chromatography: Some possible approaches for improved separations

被引：208

作者：

McCalley, David V. ^{[1
]}

机构：

[1] Univ W England, Ctr Biomed Res, Bristol BS16 1QY, Avon, England

来源：

JOURNAL OF CHROMATOGRAPHY A | 2010年 / 1217卷 / 06期

关键词：

HPLC; Basic compounds; Stationary phases; Reversed-phase; HILIC; REVERSED-PHASE COLUMNS; HYDROPHILIC-INTERACTION CHROMATOGRAPHY; POLYBUTADIENE-COATED ZIRCONIA; HYDROPHOBIC-SUBTRACTION MODEL; PRINCIPAL COMPONENT ANALYSIS; BUFFERED MOBILE PHASES; MASS-TRANSFER KINETICS; C18 STATIONARY PHASES; ADSORPTION MECHANISM; IONIZABLE COMPOUNDS;

D O I：

10.1016/j.chroma.2009.11.068

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

This review considers some of the difficulties encountered with the analysis of ionised bases using reversed-phase chromatography, such as detrimental interaction with column silanol groups, and overloading which both lead to poor peak shapes. Methods of overcoming these problems in reversed-phase (RP) separations, by judicious selection of the column and mobile phase conditions, are discussed Hydrophilic interaction chromatography is considered as an alternative method for the separation of some basic compounds. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：858 / 880

页数：23

共 95 条

[1] Radial heterogeneity of some analytical columns used in high-performance liquid chromatography [J].

Abia, Jude A. ;

Mriziq, Khaled S. ;

Guiochon, Georges A. .

JOURNAL OF CHROMATOGRAPHY A, 2009, 1216 (15) :3185-3191

[2] HYDROPHILIC-INTERACTION CHROMATOGRAPHY FOR THE SEPARATION OF PEPTIDES, NUCLEIC-ACIDS AND OTHER POLAR COMPOUNDS [J].

ALPERT, AJ .

JOURNAL OF CHROMATOGRAPHY, 1990, 499 :177-196

[3]

ANTIA FD, 1988, J CHROMATOGR, V435, P1

[4] Characterization and high performance liquid chromatographic evaluation of a new amide-functionalized reversed phase column [J].

Ascah, TL ;

Kallury, KMR ;

Szafranski, CA ;

Corman, SD ;

Liu, F .

JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES, 1996, 19 (17-18) :3049-3073

[5] SEPARATION OF ORGANIC AMINE COMPOUNDS ON SILICA-GEL WITH REVERSED-PHASE ELUENTS [J].

BIDLINGMEYER, BA ;

DELRIOS, JK ;

KORPI, J .

ANALYTICAL CHEMISTRY, 1982, 54 (03) :442-447

[6] Rationalisation of unusual changes in efficiency and retention with temperature shown for bases in reversed-phase high-performance liquid chromatography at intermediate pH [J].

Buckenmaier, SMC ;

McCalley, DV ;

Euerby, MR .

JOURNAL OF CHROMATOGRAPHY A, 2004, 1060 (1-2) :117-126

[7] Determination of ionisation constants of organic bases in aqueous methanol solutions using capillary electrophoresis [J].

Buckenmaier, SMC ;

McCalley, DV ;

Euerby, MR .

JOURNAL OF CHROMATOGRAPHY A, 2004, 1026 (1-2) :251-259

[8] Determination of pKa values of organic bases in aqueous acetonitrile solutions using capillary electrophoresis [J].

Buckenmaier, SMC ;

McCalley, DV ;

Euerby, MR .

JOURNAL OF CHROMATOGRAPHY A, 2003, 1004 (1-2) :71-79

[9] Overloading study of bases using polymeric RP-HPLC columns as an aid to rationalization of overloading on silica-ODS phases [J].

Buckenmaier, SMC ;

McCalley, DV ;

Euerby, MR .

ANALYTICAL CHEMISTRY, 2002, 74 (18) :4672-4681

[10] Kinetic plot method as a tool to design coupled column systems producing 100,000 theoretical plates in the shortest possible time [J].

Cabooter, Deirdre ;

Lestremau, Francois ;

Lynen, Frederic ;

Sandra, Pat ;

Desmet, Gert .

JOURNAL OF CHROMATOGRAPHY A, 2008, 1212 (1-2) :23-34

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