Optimization of depletion focal spot in STED nanoscopy using amplitude manipulation

被引：7
作者：
Li, Yanghui [1 ]
Lai, Lin [1 ]
Rui, Congshan [1 ]
Wang, Le [1 ]
机构：
[1] China Jiliang Univ, Coll Opt & Elect Technol, Hangzhou 310018, Zhejiang, Peoples R China
来源：
OPTICS COMMUNICATIONS | 2016年 / 372卷
基金：
中国国家自然科学基金;
关键词：
STED; Vectorial diffraction theory; Amplitude manipulation; FLUORESCENCE MICROSCOPY; DIFFRACTION; RESOLUTION; BREAKING; BARRIER; FIELD;
D O I：
10.1016/j.optcom.2016.03.081
中图分类号：
O43 [光学];
学科分类号：
070207 ; 0803 ;
摘要：
To optimize the performance of stimulated emission depletion (STED) nanoscopy, a high-quality ring shaped depletion pattern with a "zero"-intensity point at the center is mandatory. Here, we propose a novel strategy to enhance the two-dimensional (2D) resolution of STED nanoscopy by amplitude manipulation. In particular, we employ an annular mask to the depletion beam to enable a compression to the size of effective point spread function (PSF) by at least 20%. (C) 2016 Elsevier B.V. All rights reserved.
引用
收藏
页码：132 / 136
页数：5
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