Optimization of depletion focal spot in STED nanoscopy using amplitude manipulation

被引：7

作者：

Li, Yanghui ^{[1
]}

Lai, Lin ^{[1
]}

Rui, Congshan ^{[1
]}

Wang, Le ^{[1
]}

机构：

[1] China Jiliang Univ, Coll Opt & Elect Technol, Hangzhou 310018, Zhejiang, Peoples R China

来源：

OPTICS COMMUNICATIONS | 2016年 / 372卷

基金：

中国国家自然科学基金;

关键词：

STED; Vectorial diffraction theory; Amplitude manipulation; FLUORESCENCE MICROSCOPY; DIFFRACTION; RESOLUTION; BREAKING; BARRIER; FIELD;

D O I：

10.1016/j.optcom.2016.03.081

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

To optimize the performance of stimulated emission depletion (STED) nanoscopy, a high-quality ring shaped depletion pattern with a "zero"-intensity point at the center is mandatory. Here, we propose a novel strategy to enhance the two-dimensional (2D) resolution of STED nanoscopy by amplitude manipulation. In particular, we employ an annular mask to the depletion beam to enable a compression to the size of effective point spread function (PSF) by at least 20%. (C) 2016 Elsevier B.V. All rights reserved.

引用

页码：132 / 136

页数：5

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