Afatinib demonstrates remarkable activity against HER2-amplified uterine serous endometrial cancer in vitro and in vivo

被引：27

作者：

Schwab, C. L. ^{[1
]}

Bellone, S. ^{[1
]}

English, D. P. ^{[1
]}

Roque, D. M. ^{[1
]}

Lopez, S. ^{[2
]}

Cocco, E. ^{[1
]}

Nicoletti, R. ^{[1
]}

Bortolomai, I. ^{[3
]}

Bonazzoli, E. ^{[1
]}

Ratner, E. ^{[1
]}

Silasi, D-A ^{[1
]}

Azodi, M. ^{[1
]}

Schwartz, P. E. ^{[1
]}

Rutherford, T. J. ^{[1
]}

Santin, A. D. ^{[1
]}

机构：

[1] Yale Univ, Sch Med, Dept Obstet Gynecol & Reprod Sci, New Haven, CT 06520 USA

[2] Univ Campus Biomed Roma, Div Gynecol Oncol, I-00128 Rome, Italy

[3] Osped San Raffaele, Telethon Inst Gene Therapy TIGET, I-20132 Milan, Italy

来源：

BRITISH JOURNAL OF CANCER | 2014年 / 111卷 / 09期

基金：

美国国家卫生研究院;

关键词：

uterine serous cancer; HER2/Neu; afatinib; endometrial cancer; chemoresistance; tyrosine kinase inhibitors; ERBB FAMILY BLOCKER; PROGNOSTIC INDICATOR; HER-2/NEU EXPRESSION; CARCINOMA; AMPLIFICATION; EGFR; OVEREXPRESSION; MUTATIONS; CISPLATIN; PATTERNS;

D O I：

10.1038/bjc.2014.519

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Background: Uterine serous carcinomas (USCs) are an aggressive form of uterine cancer that may rely on HER2/neu amplification as a driver of proliferation. The objective of this paper is to assess the sensitivity of USC cell lines with and without HER2/neu gene amplification to afatinib, an irreversible ErbB tyrosine kinase inhibitor, and to test the efficacy of afatinib in the treatment of HER2-amplified USC xenografts. Methods: Eight of fifteen primary USC cell lines (four with HER2 amplification and four without) demonstrating similar in vitro growth rates were treated with scalar concentrations of afatinib. Effects on cell growth, signalling and cell cycle distribution were determined by flow cytometry assays. Mice harbouring xenografts of HER2/neu-amplified USC were treated with afatinib by gavage to determine the effect on tumour growth and overall survival. Results: Primary chemotherapy-resistant USC cell lines harbouring HER2/neu gene amplification were exquisitely sensitive to afatinib exposure (mean +/- s.e.m. IC50 = 0.0056 +/- 0.0006 mM) and significantly more sensitive than HER2/neu-non-amplified USC cell lines (mean +/- s.e.m. IC50 = 0.563 +/- 0.092 mM, P < 0.0001). Afatinib exposure resulted in abrogation of cell survival, inhibition of HER2/neu autophosphorylation and S6 transcription factor phosphorylation in HER2/neu overexpressing USC and inhibited the growth of HER2-amplified tumour xenografts improving overall survival (P = 0.0017). Conclusions: Afatinib may be highly effective against HER2/neu-amplified chemotherapy-resistant USC. The investigation of afatinib in patients harbouring HER2/neu-amplified USC is warranted.

引用

页码：1750 / 1756

页数：7

共 23 条

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