In Vitro Assessment of Antibacterial Activity and Cytocompatibility of Silver-Containing PHBV Nanofibrous Scaffolds for Tissue Engineering

被引:131
作者
Xing, Zhi-Cai [1 ]
Chae, Won-Pyo [1 ]
Baek, Jin-Young [1 ]
Choi, Moon-Jeong [2 ]
Jung, Yongsoo [3 ]
Kang, Inn-Kyu [1 ]
机构
[1] Kyungpook Natl Univ, Dept Polymer Sci & Engn, Taegu 702701, South Korea
[2] Kyungpook Natl Univ, Dept Med & Biol Engn, Taegu 702701, South Korea
[3] Korea Inst Mat Sci, Mat Proc Div, Chang Won 641010, South Korea
关键词
MC3T3-E1; CELLS; FIBERS; POLY(L-LACTIDE); DIFFERENTIATION; NANOPARTICLES; BEHAVIOR; HEAT; IONS; MATS;
D O I
10.1021/bm1000372
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Infections with bacteria have become a serious problem in joint arthroplasty. This study reports about in vitro antibacterial activity and in vitro cell compatibility of poly-(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) nanofibers loaded with metallic silver particles of a size of 5-13 urn. In vitro antibacterial activity against Staphylococcus aureus and Klebsiella pneumoniae was studied by microplate proliferation tests. The adhesion, viability, and proliferation properties of fibroblasts (NIH 3T3) and differentiation of osteoblasts (MC3T3-E1) were clone to study in vitro cell compatibility of the scaffolds. As the results, only silver-containing PHBV nanofibrous scaffolds showed a high antibacterial activity and an inhibitory effect on the growth of both Staphylococcus aureus and Klebsiella pneumoniae bacteria. The nanofibrous scaffolds having silver nanoparticles <1.0% were free of in vitro cytotoxicity. To sum up, the PHBV nanolibrous scaffolds having nanoparticles <1.0 wt % showed not only good antibacterial activity but also good in vitro cell compatibility. It is considered that the PHBV nanofibrous scaffolds with silver nanoparticles <1.0 wt % have a potential to be used in joint arthroplasty.
引用
收藏
页码:1248 / 1253
页数:6
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