Age and physiological condition of donor plants affect in vitro morphogenesis in leaf explants of Passiflora edulis f. flavicarpa

被引：37

作者：

Becerra, DC ^{[1
]}

Forero, AP ^{[1
]}

Góngora, GA ^{[1
]}

机构：

[1] Univ Javeriana, Dept Biol, Unidad Biotecnol Vegetal, Bogota, Colombia

来源：

PLANT CELL TISSUE AND ORGAN CULTURE | 2004年 / 79卷 / 01期

关键词：

vitro plant regeneration; maracuya; organogenesis; yellow passion fruit;

D O I：

10.1023/B:TICU.0000049440.10767.29

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A protocol for in vitro shoot regeneration from leaf discs of Passiflora edulis f. flavicarpa is described. The effect of explant source on morphogenesis was studied by comparing the response of explants collected from young plants, in vitro plantlets, and reinvigorated adult plants. Highest de novo shoot production was observed in explants from 2-month-old plants cultured in Murashige and Skoog medium supplemented with sucrose 30 g l(-1), 4.44 muM benzyladenine, and 2.32 muM kinetin. Regenerated shoots rooted spontaneously in growth regulator-free medium and were successfully acclimatized to greenhouse conditions. Influence of the age and physiological state on shoot regeneration and gradual loss of morphogenic capacity is discussed.

引用

页码：87 / 90

页数：4

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