Atmospheric pressure chemical ionisation liquid chromatography/mass spectrometry of type II chlorophyll-a transformation products: Diagnostic fragmentation patterns

被引：14

作者：

Bale, Nicole J. ^{[1
]}

Llewellyn, Carole A. ^{[1
]}

Airs, Ruth L. ^{[1
]}

机构：

[1] Plymouth Marine Lab, Plymouth PL1 3DH, Devon, England

来源：

ORGANIC GEOCHEMISTRY | 2010年 / 41卷 / 05期

关键词：

CHLORELLA-PROTOTHECOIDES; MASS-SPECTROMETRY; CATABOLITE REDUCTASE; RED PIGMENTS; BREAKDOWN; SEDIMENTS; ELUCIDATION; DEGRADATION; DERIVATIVES; CHLORIN;

D O I：

10.1016/j.orggeochem.2010.01.007

中图分类号：

P3 [地球物理学]; P59 [地球化学];

学科分类号：

0708 ; 070902 ;

摘要：

Atmospheric pressure chemical ionisation liquid chromatography/multistage mass spectrometry (APCI LC/MS(n)) is an ideal tool for the analysis of complex mixtures of natural compounds produced in the aquatic environment. In contrast to extensive reports of open chain (type II) chlorophyll (chl) transformation products (also known as chl catabolites) in terrestrial plants, there have been no reports relating to aquatic equivalents. This study examines the fragmentation pathways of type II chl transformation products from APCI LC/MS(n) analysis in order to determine whether it is a suitable analytical method for detecting and distinguishing among such compounds if they are present in samples from the aquatic environment. An extract from the medium of a laboratory culture of the alga Chlorella protothecoides was analysed and found to contain a number of structurally similar compounds assigned as being either red chlorophyll catabolites (RCCs) or fluorescent chlorophyll catabolites (FCCs). The five structures examined were found to exhibit one of three distinct fragmentation patterns, a result that highlights the potential of APCI LC/MS(n) for distinguishing among different type II chl transformation products. (C) 2010 Elsevier Ltd. All rights reserved.

引用

页码：473 / 481

页数：9

共 31 条

[1] A novel sedimentary chlorin: structural evidence for a chlorophyll origin for aetioporphyrins [J].

Airs, RL ;

Jie, C ;

Keely, BJ .

ORGANIC GEOCHEMISTRY, 2000, 31 (11) :1253-1256

[2] Atmospheric pressure chemical ionisation liquid chromatography/mass spectrometry of bacteriochlorophylls from Chlorobiaceae: characteristic fragmentations [J].

Airs, RL ;

Keely, BJ .

RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 2002, 16 (05) :453-461

[3] Development and application of a high resolution liquid chromatographic method for the analysis of complex pigment distributions [J].

Airs, RL ;

Atkinson, JE ;

Keely, BJ .

JOURNAL OF CHROMATOGRAPHY A, 2001, 917 (1-2) :167-177

[4]

BROWN SB, 1991, CHLOROPHYLLS, P39

[5] Implications for the role of pre- versus post-depositional transformation of chlorophyll-a in the Lower Mississippi River and Louisiana shelf [J].

Chen, N ;

Bianchi, TS ;

Bland, JM .

MARINE CHEMISTRY, 2003, 81 (1-2) :37-55

[6] EVIDENCE FOR A MONOOXYGENASE-CATALYZED PRIMARY PROCESS IN THE CATABOLISM OF CHLOROPHYLL [J].

CURTY, C ;

ENGEL, N ;

GOSSAUER, A .

FEBS LETTERS, 1995, 364 (01) :41-44

[7]

Engel N, 1996, PLANT PHYSIOL BIOCH, V34, P77

[8] CHLOROPHYLL CATABOLISM IN CHLORELLA-PROTOTHECOIDES - ISOLATION AND STRUCTURE ELUCIDATION OF A RED BILIN DERIVATIVE [J].

ENGEL, N ;

JENNY, TA ;

MOOSER, V ;

GOSSAUER, A .

FEBS LETTERS, 1991, 293 (1-2) :131-133

[9] Novel carotenol chlorin esters in marine sediments and water column particulate matter [J].

Goericke, R ;

Shankle, A ;

Repeta, DJ .

GEOCHIMICA ET COSMOCHIMICA ACTA, 1999, 63 (18) :2825-2834

[10] IDENTIFICATION OF CHLOROPHYLL TRANSFORMATION PRODUCTS IN ZOOPLANKTON FECAL PELLETS AND MARINE SEDIMENT EXTRACTS BY LIQUID-CHROMATOGRAPHY MASS-SPECTROMETRY ATMOSPHERIC-PRESSURE CHEMICAL-IONIZATION [J].

HARRIS, PG ;

CARTER, JF ;

HEAD, RN ;

HARRIS, RP ;

EGLINTON, G ;

MAXWELL, JR .

RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 1995, 9 (12) :1177-1183

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