Local delivery of deep marine fungus-derived equisetin from polyvinylpyrrolidone (PVP) nanofibers for anti-MRSA activity

被引:22
作者
Luo, Minghe [1 ]
Ming, Yue [1 ]
Wang, Linli [1 ]
Li, Yuanyuan [1 ]
Li, Bin [1 ]
Chen, Jianhong [1 ]
Shi, Sanjun [1 ]
机构
[1] Third Mil Med Univ, Inst Surg Res, Daping Hosp, Dept Pharm, Chongqing 400042, Peoples R China
基金
中国国家自然科学基金;
关键词
MRSA; Equisetin; PVP nanofibers; Marine fungus; Skin and soft tissue infections; RESISTANT STAPHYLOCOCCUS-AUREUS; BIOMEDICAL APPLICATIONS; WOUND DRESSINGS; ELECTROSPUN; CANCER; SKIN; SCAFFOLDS; INFECTION; RELEASE; SYSTEM;
D O I
10.1016/j.cej.2018.05.159
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
Methicillin-resistant Staphylococcus aureus (MRSA) is emerging as a serious pathogenic bacterium in the clinical setting, and antibiotic resistance is spreading faster than the introduction of new agents to kill such bacteria. In our study, Fusarium sp. 152, a marine fungus obtained from the South China Sea, was fermented to screen new anti-MRSA compounds including equisetin (EQ) with a MIC value of 1 mu g/mL. Then, EQ was loaded into nanoparticle- based polyvinylpyrrolidone (PVP) electrospinning to facilitate its practical use. After electrospinning, the EQ nanofibers (EQ/NPs nanofiber) were 374.9 nm in diameter, and they showed a good flexibility and foldability. Additionally, the EQ/NPs nanofibers showed a better antibacterial effect both in vitro and in vivo than cefoxitin. Simultaneously, the infectious wounds treated with EQ/NPs nanofiber healed better and faster than those treated with EQ and cefoxitin. These findings strongly demonstrate that PVP-based electrospinning dressings are a candidate delivery carrier for the new anti-MRSA compound EQ for topical therapy.
引用
收藏
页码:157 / 163
页数:7
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