Genetic and biochemical studies of phosphatase activity of PhoR

被引:49
作者
Carmany, DO [1 ]
Hollingsworth, K [1 ]
McCleary, WR [1 ]
机构
[1] Brigham Young Univ, Microbiol & Mol Biol Dept, Provo, UT 84602 USA
关键词
D O I
10.1128/JB.185.3.1112-1115.2003
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
In Escherichia coli, PhoR is the histidine kinase of the phosphate regulon. It has been postulated that PhoR may function as a phospho-PhoB phosphatase. Experiments with four precise phoR deletion mutants supported this hypothesis and suggested that this activity resides within the histidine phosphorylation domain. This biochemical activity was confirmed by using a separately expressed histidine phosphorylation domain.
引用
收藏
页码:1112 / 1115
页数:4
相关论文
共 26 条
[1]   CROSS TALK TO THE PHOSPHATE REGULON OF ESCHERICHIA-COLI BY PHOM PROTEIN - PHOM IS A HISTIDINE PROTEIN-KINASE AND CATALYZES PHOSPHORYLATION OF PHOB AND PHOM-OPEN READING FRAME-2 [J].
AMEMURA, M ;
MAKINO, K ;
SHINAGAWA, H ;
NAKATA, A .
JOURNAL OF BACTERIOLOGY, 1990, 172 (11) :6300-6307
[2]  
[Anonymous], 1996, Escherichia coli and Salmonella: cellular and molecular biology
[3]   Autophosphorylation, phosphotransfer, and DNA-binding properties of the RegB RegA two-component regulatory system in Rhodobacter capsulatus [J].
Bird, TH ;
Du, SY ;
Bauer, CE .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (23) :16343-16348
[4]   One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products [J].
Datsenko, KA ;
Wanner, BL .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (12) :6640-6645
[5]   Histidine kinases: diversity of domain organization [J].
Dutta, R ;
Qin, L ;
Inouye, M .
MOLECULAR MICROBIOLOGY, 1999, 34 (04) :633-640
[6]  
Hoch J.A., 1995, Two-component Signal Transduction
[7]   ENVZ, A TRANSMEMBRANE ENVIRONMENTAL SENSOR OF ESCHERICHIA-COLI K-12, IS PHOSPHORYLATED INVITRO [J].
IGO, MM ;
SILHAVY, TJ .
JOURNAL OF BACTERIOLOGY, 1988, 170 (12) :5971-5973
[8]   REGULATION OF THE PHOSPHATE REGULON OF ESCHERICHIA-COLI - CHARACTERIZATION OF THE PROMOTER OF THE PSTS GENE [J].
KIMURA, S ;
MAKINO, K ;
SHINAGAWA, H ;
AMEMURA, M ;
NAKATA, A .
MOLECULAR & GENERAL GENETICS, 1989, 215 (03) :374-380
[9]   ROLE OF THE SIGMA-70-SUBUNIT OF RNA-POLYMERASE IN TRANSCRIPTIONAL ACTIVATION BY ACTIVATOR PROTEIN PHOB IN ESCHERICHIA-COLI [J].
MAKINO, K ;
AMEMURA, M ;
KIM, SK ;
NAKATA, A ;
SHINAGAWA, H .
GENES & DEVELOPMENT, 1993, 7 (01) :149-160
[10]   REGULATION OF THE PHOSPHATE REGULON OF ESCHERICHIA-COLI - ACTIVATION OF PSTS TRANSCRIPTION BY PHOB PROTEIN INVITRO [J].
MAKINO, K ;
SHINAGAWA, H ;
AMEMURA, M ;
KIMURA, S ;
NAKATA, A ;
ISHIHAMA, A .
JOURNAL OF MOLECULAR BIOLOGY, 1988, 203 (01) :85-95