Important role of loops in the transport activity of the mitochondrial ADP/ATP carrier

被引：19

作者：

Terada, H ^{[1
]}

Majima, E ^{[1
]}

机构：

[1] Univ Tokushima, Fac Pharmaceut Sci, Tokushima 770, Japan

来源：

FORMATION AND DYNAMICS OF SELF-ORGANIZED STRUCTURES IN SURFACTANTS AND POLYMER SOLUTIONS | 1997年 / 106卷

关键词：

ADP/ATP carrier; loop structure; substrate binding site; SH-reagent; mitochondria;

D O I：

10.1007/BF01189519

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

The transport mechanism of the bovine heart mitochondrial ADP/ATP carrier was studied using submitochondrial particles. The modifications of the cysteine residues of the carrier by the SH-reagents eosin-5-maleimide (EMA) and N-ethylmaleimide (NEM). and disulfide bond formation between these cysteine residues catalyzed by copper-o-phenanthroline (Cu(OP)(2)) under various conditions were studied. In particular. the effects of the transport inhibitors carboxyatractyloside (CATR) and bongkrekic acid (BKA), and fluorescein derivatives were examined. From the results, the topology of the carrier in the membrane, dynamic translocations of the loops of the carrier, and the structure of the primary binding site of the transport substrates ADP and ATP were deduced. The loops are concluded to act as both gates in the transport and binding sites for the substrates. Based on the results. a cooperative swinging-loop model is postulated as the transport mechanism of the ADP:ATP carrier.

引用

页码：192 / 197

页数：6

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