Fructose-Based Production of Short-Chain-Length and Medium-Chain-Length Polyhydroxyalkanoate Copolymer by Arctic Pseudomonas sp. B14-6

被引：38
作者：
Choi, Tae-Rim [1 ]
Park, Ye-Lim [1 ]
Song, Hun-Suk [1 ]
Lee, Sun Mi [1 ]
Park, Sol Lee [1 ]
Lee, Hye Soo [1 ]
Kim, Hyun-Joong [1 ]
Bhatia, Shashi Kant [1 ]
Gurav, Ranjit [1 ]
Choi, Kwon-Young [3 ]
Lee, Yoo Kyung [4 ]
Yang, Yung-Hun [1 ,2 ]
机构：
[1] Konkuk Univ, Coll Engn, Dept Biol Engn, Seoul 05029, South Korea
[2] Konkuk Univ, Inst Ubiquitous Informat Technol & Applicat, Seoul 05029, South Korea
[3] Ajou Univ, Coll Engn, Dept Environm & Safety Engn, Suwon 16499, South Korea
[4] Polar Res Inst, Incheon 21990, South Korea
来源：
POLYMERS | 2021年 / 13卷 / 09期
基金：
新加坡国家研究基金会;
关键词：
Pseudomonas strain; polyhydroxyalkanoate; fructose syrup; fermentation; BACTERIAL POLYHYDROXYALKANOATES; CARBON-SOURCES; BIOSYNTHESIS; ACID; POLY(3-HYDROXYBUTYRATE); CONVERSION; POLYMERS; GENES;
D O I：
10.3390/polym13091398
中图分类号：
O63 [高分子化学（高聚物）];
学科分类号：
070305 ; 080501 ; 081704 ;
摘要：
Arctic bacteria employ various mechanisms to survive harsh conditions, one of which is to accumulate carbon and energy inside the cell in the form of polyhydroxyalkanoate (PHA). Whole-genome sequencing of a new Arctic soil bacterium Pseudomonas sp. B14-6 revealed two PHA-production-related gene clusters containing four PHA synthase genes (phaC). Pseudomonas sp. B14-6 produced poly(6% 3-hydroxybutyrate-co-94% 3-hydroxyalkanoate) from various carbon sources, containing short-chain-length PHA (scl-PHA) and medium-chain-length PHA (mcl-PHA) composed of various monomers analyzed by GC-MS, such as 3-hydroxybutyrate, 3-hydroxyhexanoate, 3-hydroxyoctanoate, 3-hydroxydecanoate, 3-hydroxydodecenoic acid, 3-hydroxydodecanoic acid, and 3-hydroxytetradecanoic acid. By optimizing the PHA production media, we achieved 34.6% PHA content using 5% fructose, and 23.7% PHA content using 5% fructose syrup. Differential scanning calorimetry of the scl-co-mcl PHA determined a glass transition temperature (T-g) of 15.3 degrees C, melting temperature of 112.8 degrees C, crystallization temperature of 86.8 degrees C, and 3.82% crystallinity. In addition, gel permeation chromatography revealed a number average molecular weight of 3.6 x 10(4), weight average molecular weight of 9.1 x 10(4), and polydispersity index value of 2.5. Overall, the novel Pseudomonas sp. B14-6 produced a polymer with high medium-chain-length content, low T-g, and low crystallinity, indicating its potential use in medical applications.
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页数：14
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