Structure of the mitochondrial β-ketoacyl-[acyl carrier protein] synthase from Arabidopsis and its role in fatty acid synthesis

Mitochondrial fatty acid synthesis is catalyzed by a dissociated fatty acid synthase similar to those of plant plastids and bacteria. The crystal structure of a mitochondrial beta-ketoacyl-[acyl carrier protein] synthase (mtKAS), namely that from Arabidopsis thaliana, has been determined for the first time. This enzyme accomplishes the vital condensation steps in constructing fatty acid carbon skeletons. The product profile of mtKAS is unusual in that C-8 and C14-16 fatty acyl chains predominate. An enzyme architecture that likely is the basis for the observed bimodal profile of mtKAS products can be derived from the shape of the acyl binding pocket. (C) 2004 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.