Preparation and properties of EDC/NHS mediated crosslinking poly (gamma-glutamic acid)/epsilon-polylysine hydrogels

被引：148
作者：
Hua, Jiachuan [1 ,2 ]
Li, Zheng [1 ,2 ]
Xia, Wen [1 ,2 ]
Yang, Ning [1 ,2 ]
Gong, Jixian [1 ,2 ]
Zhang, Jianfei [1 ,2 ]
Qiao, Changsheng [3 ]
机构：
[1] Tianjin Polytech Univ, Minist Educ, Key Lab Adv Text Composites, Tianjin 300387, Peoples R China
[2] Tianjin Polytech Univ, Sch Text, Tianjin 300387, Peoples R China
[3] Tianjin Univ Sci & Technol, Minist Educ, Key Lab Ind Microbiol, Tianjin 300457, Peoples R China
来源：
MATERIALS SCIENCE AND ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS | 2016年 / 61卷
基金：
中国国家自然科学基金;
关键词：
Poly gamma-glutamic acid; epsilon-Polylysine; Hydrogel; Swelling kinetics; Biocompatibility; MICROBIAL POLY(GAMMA-GLUTAMIC ACID); L-LYSINE; MECHANICAL-PROPERTIES; POLY(ASPARTIC ACID); DRUG-DELIVERY; DEGRADATION; PERFORMANCE; POLYLYSINE; COLLAGEN;
D O I：
10.1016/j.msec.2016.01.001
中图分类号：
TB3 [工程材料学]; R318.08 [生物材料学];
学科分类号：
0805 ; 080501 ; 080502 ;
摘要：
In this paper, a novel pH-sensitive poly (amino acid) hydrogel based on poly gamma-glutamic acid (gamma-PGA) and epsilon-polylysine (epsilon-PL) was prepared by carbodiimide (EDC) and N-hydroxysuccinimide (NHS) mediated polymerization. The influence of PGA/PL molar ratio and EDC/NHS concentration on the structure and properties was studied. Fourier transform infrared spectroscopy (FT-IR) and X-ray photoelectron spectroscopy (XPS) proved that hydrogels were crosslinked through amide bond linkage, and the conversion rate of a carboxyl group could reach 96%. Scanning electron microscopy (SEM) results showed a regularly porous structure with 20 mu m pore size in average. The gelation time in the crosslink process of PGA/PL hydrogels was within less than 5 min. PGA/PL hydrogels had excellent optical performance that was evaluated by a novel optotype method. Furthermore, PGA/PL hydrogels were found to be pH sensitive, which could be adjusted to the pH of swelling media intelligently. The terminal pH of swelling medium could be controlled at 5 +/- 1 after equilibrium when the initial pH was within 3-11. The swelling kinetics was found to follow a Voigt model in deionized water but a pseudo-second-order model in normal saline and phosphate buffer solution, respectively. The differential swelling degrees were attributed to the swelling theory based on the different ratio of -COOH/NH2 and pore size in hydrogels. The results of mechanical property indicated that PGA/PL hydrogels were soft and elastic. Moreover, PGA/PL hydrogels exhibited excellent biocompatibility by cell proliferation experiment. PGA/PL hydrogels could be degraded in PBS solution and the degradation rate was decreased with the increase of the molar ratio of PL. Considering the simple preparation process and pH-sensitive property, these PGA/PL hydrogels might have high potential for use in medical and clinical fields. (C) 2016 Elsevier B.V. All rights reserved.
引用
收藏
页码：879 / 892
页数：14
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