Detection of Bean yellow mosaic potyvirus in infected faba bean plants (Vicia faba L.) from Northern Egypt

The Bean yellow mosaic potyvirus (BYMV) was isolated from naturally infected faba bean plants (Vicia faba L.) showing different symptoms of mosaic, mottle, and vein clearing, grown at certain locations of El Beheira governorate, Egypt, during the growing seasons from 2018- 2019. BYMV isolate was transmitted by Acyrthosiphon pisum, Aphis faba, A. nerii Boyer, Brevicoryne brassicae and Dactynotus sonchi L. in non-persistent manner. The virus was partially purified using polyethylene glycol PEG 6000, and differential centrifugation. The ratio of A 260/280 was 1.47. Whereas, the ratio of A max/min was 0.93. Concentration of the virus in the preparation was estimated using an extinction coefficient of E-0.1%(260) = 2.8 Yield of purified virus was about 14.8 mg/100g fresh weight of leaves of Vicia faba. Antiserum titer was determined by indirect enzyme linked immunosorbent assay (ELISA). Positive ELISA values were obtained up to dilutions of 1: 256000. The virus was detected by indirect ELISA in infected sap at 8 and 16 days after inoculation and by tissue blot immunoassay (TBIA) and dot blot immunoassay (DBIA) on nitrocellulose membrane after the same period. The unused face of the processed nitrocellulose membrane already printed with plant tissues revealed that both faces of nitrocellulose membrane and old processed nitrocellulose membrane could be used as solid carriers in TBIA, for detection of BYMV in infected leaves.