Hemopressins and other hemoglobin-derived peptides in mouse brain: comparison between brain, blood, and heart peptidome and regulation in Cpefat/fat mice

被引：47

作者：

Gelman, Julia S. ^{[2
]}

Sironi, Juan ^{[1
]}

Castro, Leandro M. ^{[3
]}

Ferro, Emer S. ^{[3
]}

Fricker, Lloyd D. ^{[1
,2
]}

机构：

[1] Albert Einstein Coll Med, Dept Mol Pharmacol, Bronx, NY 10461 USA

[2] Albert Einstein Coll Med, Dominic P Purpura Dept Neurosci, Bronx, NY 10461 USA

[3] Univ Sao Paulo, Dept Cell Biol & Dev, BR-05508 Sao Paulo, Brazil

来源：

JOURNAL OF NEUROCHEMISTRY | 2010年 / 113卷 / 04期

基金：

美国国家卫生研究院;

关键词：

cannabinoid; carboxypeptidase E; hemoglobin; neuropeptide; peptide processing; ENKEPHALIN-SYNTHESIZING CARBOXYPEPTIDASE; PURKINJE-CELL DEGENERATION; STABLE ISOTOPIC TAGS; OBESE FAT/FAT MICE; CPE(FAT)/CPE(FAT) MICE; MASS-SPECTROMETRY; NERVOUS-SYSTEM; PITUITARY; IDENTIFICATION; EXPRESSION;

D O I：

10.1111/j.1471-4159.2010.06653.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

P>Many hemoglobin-derived peptides are present in mouse brain, and several of these have bioactive properties including the hemopressins, a related series of peptides that bind to cannabinoid CB1 receptors. Although hemoglobin is a major component of red blood cells, it is also present in neurons and glia. To examine whether the hemoglobin-derived peptides in brain are similar to those present in blood and heart, we used a peptidomics approach involving mass spectrometry. Many hemoglobin-derived peptides are found only in brain and not in blood, whereas all hemoglobin-derived peptides found in heart were also seen in blood. Thus, it is likely that the majority of the hemoglobin-derived peptides detected in brain are produced from brain hemoglobin and not erythrocytes. We also examined if the hemopressins and other major hemoglobin-derived peptides were regulated in the Cpefat/fat mouse; previously these mice were reported to have elevated levels of several hemoglobin-derived peptides. Many, but not all of the hemoglobin-derived peptides were elevated in several brain regions of the Cpefat/fat mouse. Taken together, these findings suggest that the post-translational processing of alpha and beta hemoglobin into the hemopressins, as well as other peptides, is up-regulated in some but not all Cpefat/fat mouse brain regions.

引用

页码：871 / 880

页数：10

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