Role of GPI-anchored NCAM-120 in rabies virus infection

被引：15

作者：

Hotta, Kozue

Motoi, Yurie

Okutani, Akiko

Kaku, Yoshihiro

Noguchi, Akira

Inoue, Satoshi

Yamada, Akio

机构：

[1] Natl Inst Infect Dis, Dept Vet Sci, Shinjuku Ku, Tokyo 1628640, Japan

[2] Gifu Univ, United Grad Sch Vet Sci, Gifu 5011193, Japan

[3] Meat Inspect Lab, Sawa, Gunma 3701103, Japan

来源：

MICROBES AND INFECTION | 2007年 / 9卷 / 02期

关键词：

rabies virus; receptor; neural cell adhesion molecule; interferon beta;

D O I：

10.1016/j.micinf.2006.11.003

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Although the neural cell adhesion molecule (NCAM) -140 and -180 have been shown to serve as a receptor for rabies virus (RV), it was not known whether the other major isoform of NCAM, GPI-anchored NCAM-120 functions as RV receptor. In this study, we have established HEp2 cells stably expressing NCAM-120 or NCAM-140, and their susceptibilities to RV infection were compared. The results demonstrated that NCAM-120 served as virus attachment protein; however, the cells expressing NCAM-120 did not support efficient RV replication. Furthermore, the level of IFN-beta mRNA was apparently elevated in NCAM-120 expressing cells but not in NCAM-140 expressing cells, suggesting that GPIanchored NCAM-120 suppressed RV replication via induction of IFN-beta even though NCAM-120 was able to promote virus penetration into the cells. (c) 2006 Elsevier Masson SAS. All rights reserved.

引用

页码：167 / 174

页数：8

共 30 条

[1] DIFFERENTIAL SPLICING AND ALTERNATIVE POLYADENYLATION GENERATES DISTINCT NCAM TRANSCRIPTS AND PROTEINS IN THE MOUSE [J].