Cell Heterogeneity Uncovered by Single-Cell RNA Sequencing Offers Potential Therapeutic Targets for Ischemic Stroke

被引：22

作者：

Qiu, Min ^{[1
]}

Zong, Jia-bin ^{[1
]}

He, Quan-wei ^{[1
]}

Liu, Yu-xiao ^{[1
]}

Wan, Yan ^{[1
]}

Li, Man ^{[1
]}

Zhou, Yi-fan ^{[1
]}

Wu, Jie-hong ^{[1
]}

Hu, Bo ^{[1
]}

机构：

[1] Huazhong Univ Sci & Technol, Union Hosp, Tongji Med Coll, Dept Neurol, Wuhan 430022, Peoples R China

来源：

AGING AND DISEASE | 2022年 / 13卷 / 05期

基金：

中国国家自然科学基金;

关键词：

single-cell RNA sequencing; ischemic stroke; cellular heterogeneity; differentially expressed genes; BLOOD-BRAIN-BARRIER; OLIGODENDROCYTE PROGENITOR CELLS; CENTRAL-NERVOUS-SYSTEM; REGULATORY T-CELLS; NEUROVASCULAR UNIT; REACTIVE ASTROCYTES; COGNITIVE DECLINE; MICROGLIA; DYSFUNCTION; DISEASE;

D O I：

10.14336/AD.2022.0212

中图分类号：

R592 [老年病学]; C [社会科学总论];

学科分类号：

03 ; 0303 ; 100203 ;

摘要：

Ischemic stroke is a detrimental neurological disease characterized by an irreversible infarct core surrounded by an ischemic penumbra, a salvageable region of brain tissue. Unique roles of distinct brain cell subpopulations within the neurovascular unit and peripheral immune cells during ischemic stroke remain elusive due to the heterogeneity of cells in the brain. Single-cell RNA sequencing (scRNA-seq) allows for an unbiased determination of cellular heterogeneity at high-resolution and identification of cell markers, thereby unveiling the principal brain clusters within the cell-type-specific gene expression patterns as well as cell-specific subclusters and their functions in different pathways underlying ischemic stroke. In this review, we have summarized the changes in differentiation trajectories of distinct cell types and highlighted the specific pathways and genes in brain cells that are impacted by stroke. This review is expected to inspire new research and provide directions for investigating the potential pathological mechanisms and novel treatment strategies for ischemic stroke at the level of a single cell.

引用

收藏

页码：1436 / 1454

页数：19

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