Reversible immobilization of laccase to poly(4-vinylpyridine) grafted and Cu(II) chelated magnetic beads: Biodegradation of reactive dyes

被引：98

作者：

Bayramoglu, Guelay ^{[1
]}

Yilmaz, Meltem ^{[2
]}

Arica, M. Yakup ^{[1
]}

机构：

[1] Gazi Univ, Fac Arts & Sci, Biochem Proc & Biomat Res Lab, TR-06500 Ankara, Turkey

[2] Gazi Univ, Inst Sci & Technol, Dept Environm Sci, TR-06570 Ankara, Turkey

来源：

BIORESOURCE TECHNOLOGY | 2010年 / 101卷 / 17期

关键词：

Laccase; Immobilized enzyme; Biodegradation; Reactive dyes; Decolorization; REMOVAL; DEGRADATION; OXIDATION; SYSTEMS;

D O I：

10.1016/j.biortech.2010.03.088

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

Poly(4-vinylpyridine), poly(VP), as a novel metal-chelating fibrous polymer was grafted on the magnetic beads. Poly(4-VP) grafted and/or Cu(II) ions chelated magnetic beads were used for reversible immobilization of Trametes versicolor laccase, and the amounts of immobilized laccase on the beads were determined as 36.8 and 56.4 mg/g beads, respectively. The adsorption of laccase on both modified magnetic beads appeared to follow the Langmuir isotherm model. The degradation of textile dyes with immobilized laccase on the metal chelated magnetic beads was evaluated in a batch system. Three different reactive textile dyes (i.e., Reactive Green 19, Reactive Red 2 and Reactive Brown 10) were successfully degraded in the enzyme reactor. It was observed that the decolorization rate varied widely with chemical structure and types of the substitute group of the reactive dye molecules. (C) 2010 Elsevier Ltd. All rights reserved.

引用

页码：6615 / 6621

页数：7

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