In vitro induction of neuronal apoptosis by anti-Fas antibody-containing sera from amyotrophic lateral sclerosis patients

被引:66
作者
Yi, FH
Lautrette, C
Vermot-Desroches, C
Bordessoule, D
Couratier, P
Wijdenes, J
Preud'homme, JL
Jauberteau, MO
机构
[1] Univ Hosp, Immunol Lab, CNRS EP 118, F-87042 Limoges, France
[2] Univ Hosp, Immunol Lab, CNRS ESA 6031, F-86021 Poitiers, France
[3] Diaclone, F-25020 Besancon, France
[4] Univ Hosp, Dept Hematol, F-87042 Limoges, France
[5] Univ Hosp, Dept Neurol, F-87042 Limoges, France
关键词
apoptosis; motoneurons; Fas; amyotrophic lateral sclerosis; Alzheimer disease; autoantibodies;
D O I
10.1016/S0165-5728(00)00288-5
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Sera from 26% Of patients with sporadic amyotrophic lateral sclerosis (ALS) induced in vitro apoptosis of a human neuroblastoma cell line, as detected by two methods, and most contained anti-Fas autoantibodies. in contrast, Alzheimer sera (studied as controls) very rarely induced apoptosis and did not contain detectable anti-Fas antibodies. Soluble Fas-ligand levels in ALS sera were not different from those in normal sera, except for slightly higher levels in a single case. In mixed cultures of rat embryonic brain and spinal cord cells, ALS sera (and agonistic anti-fas monoclonal antibodies and soluble Fas-ligand) induced the apoptosis of a subpopulation of neurons. These neurons were motoneurons on the basis of staining with the monoclonal antibody SMI 32 and Fas expression was restricted to these SMI 32-positive neurons. These data are compatible with the hypothesis of the participation of an autoimmune mechanism possibly related to anti-Fas autoantibodies in certain ALS patients. (C) 2000 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:211 / 220
页数:10
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