Modification of the deoxyribose test to detect strong iron binding

被引：10

作者：

Sadowska-Bartosz, Izabela ^{[1
]}

Galiniak, Sabina ^{[1
]}

Bartosz, Grzegorz ^{[1
,2
]}

机构：

[1] Univ Rzeszow, Fac Biol & Agr, Dept Biochem & Cell Biol, Rzeszow, Poland

[2] Univ Lodz, Fac Biol & Environm Protect, Dept Mol Biophys, Lodz, Poland

来源：

ACTA BIOCHIMICA POLONICA | 2017年 / 64卷 / 01期

关键词：

chelation; deoxyribose test; desferrioxamine; DETAPA EDTA; Fenton reaction; hydrogen peroxide; hydroxyl radical; iron; superoxide; HYDROXYL RADICALS; ANTIOXIDANT ACTIVITY; LIPID-PEROXIDATION; RATE CONSTANTS; ASSAY; DEGRADATION; PRODUCTS; ACID;

D O I：

10.18388/abp.2016_1385

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Deoxyribose test has been widely used for determination of reactivities of various compounds for the hydroxyl radical. The test is based on the formation of hydroxyl radical by Fe2+ complex in the Fenton reaction. We propose a modification of the deoxyribose test to detect strong iron binding, inhibiting participation of Fe2+ in the Fenton reaction, on the basis of examination of concentration dependence of deoxyribose degradation on Fe2+ concentration, at a constant concentration of a chelating agent.

引用

页码：195 / 198

页数：4

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