Evaluation of culture conditions of locally isolated Dunaliella salina strain EgeMacc-024

The aim of this study was to optimize physical parameters such as light intensity, temperature and agitation rate which might affect the cultivation of Dunaliella sauna strain EgeMacc-024. For this purpose, a set of experiments were designed by central composite design using response surface methodology to statistically evaluate the findings. The optimal growth conditions were found at 22.09 degrees C under the light intensity of 55.75 mu mol photons m(-2) s(-1) at the agitation rate of 148.73 rpm with the chlorophyll-a concentration of 6.47 mg/L, whereas the protein amount was 14.87 mu g/100 mu L at 27.97 degrees C under the light intensity of 57.5 mu mol photons m(-2) s(-1) with the agitation rate of 166.08 rpm for D. salina. The maximum specific growth rate of 0.22 day(-1), which corresponded to the doubling time of 3.15 day, was obtained for D. salina under determined conditions. (C) 2014 Elsevier B.V. All rights reserved.