Multiple levels of interactions within the tetraspanin web

被引：115

作者：

Charrin, S

Manié, S

Billard, M

Ashman, L

Gerlier, D

Boucheix, C

Rubinstein, E ^{[1
]}

机构：

[1] Hop Paul Brousse, Inst Andre Lwoff, INSERM, U268, F-94807 Villejuif, France

[2] Fac Med, Genet Lab, UMR 5641, Lyon, France

[3] Univ Newcastle, Sch Biomed Sci, Callaghan, NSW 2308, Australia

[4] UCBL, CNRS, UMR 5537, Immunite & Infect Virales Virol & Pathogenese Vir, Lyon, France

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2003年 / 304卷 / 01期

关键词：

tetraspanin; CD9; CD151; integrin; interaction; detergent-resistant membrane;

D O I：

10.1016/S0006-291X(03)00545-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The tetraspanin web refers to a network of molecular interactions involving tetraspanins and other molecules. Inside the tetraspanin web, small primary complexes containing only one tetraspanin and one specific partner molecule such as CD151/alpha3beta1 integrin and CD9/CD9P-1 (FPRP) can be observed under particular conditions. Here we demonstrate that when cells are lysed with Brij97, the tetraspanins CD 151 and CD9 allow and/or stabilize the interaction of their partner molecules with other tetraspanins and that their two partners associate under conditions maintaining tetraspanin/tetraspanin interactions. The tetraspanins were also found to partition into a detergent-resistant membrane environment to which the integrin alpha3beta1 was relocalized upon expression of CD151. (C) 2003 Elsevier Science (USA). All rights reserved.

引用

页码：107 / 112

页数：6

共 46 条

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