NtCOMT1 responsible for phytomelatonin biosynthesis confers drought tolerance in Nicotiana tabacum

被引:11
作者
Yao, Zhengping [1 ,2 ]
Zhang, Xue [1 ,2 ]
Liang, Yingchong [1 ,2 ]
Zhang, Jiemei [1 ,2 ]
Xu, Yi [4 ]
Chen, Suiyun [1 ,3 ]
Zhao, Dake [1 ,3 ]
机构
[1] Yunnan Univ, Biocontrol Engn Res Ctr Plant Dis & Pest, Biocontrol Engn Res Ctr Crop Dis & Pest, Kunming, Peoples R China
[2] Yunnan Univ, Sch Life Sci, Kunming, Peoples R China
[3] Yunnan Univ, Sch Ecol & Environm Sci, Kunming, Peoples R China
[4] Yunnan Baiyao Grp Co Ltd, Yunnan Inst Mat Med, Kunming, Peoples R China
基金
中国国家自然科学基金;
关键词
Nicotiana tabacum; Solanaceae; Melatonin; Caffeic acid O -Methyltransferase (COMT); In vitro enzymic catalyzation; Drought stress; ACID O-METHYLTRANSFERASE; BIOLOGICAL FUNCTIONS; N-ACETYLSEROTONIN; GENE-EXPRESSION; MELATONIN; STRESS; METABOLISM; PLANT; ARABIDOPSIS; RESPONSES;
D O I
10.1016/j.phytochem.2022.113306
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Nicotiana tabacum (tobacco) is one of the most important industrial crops and its productivity is vulnerable to drought, particularly in Yunnan province, China due to the long water-deficit spring. Here, we aimed at iden-tifying caffeic acid O-methyltransferase (COMT) in melatonin biosynthesis to provide genetic resources against drought tolerance of tobacco. The integration of the genome-wide identification, phylogenetic relationships, and conserved domain/motif analysis revealed that NtCOMT1 could be the probable functional COMT homolog for melatonin production. In vitro enzyme activity test approved that NtCOMT1 enabled the conversion of N-ace-tylserotonin into melatonin, occurring both in the cytoplasm and nucleus by subcellular localization analysis. The Km and Vmax values for NtCOMT1 at the optimum temperature (30 C) were 266.0 mu M and 2.155 nmol/min/ mg protein. NtCOMT1 was significantly induced by drought stress; whereby if this gene functioned on promoting drought resistance was further conducted. Overexpression of NtCOMT1 resulted in decreased wilting in trans -genic tobacco plants subjected to dehydration treatment. The combinatorial effects of NtCOMT1 in increasing melatonin content, inducing antioxidant system, and elevating the expression of drought-related genes could deliver the drought tolerance in tobacco. The characterization of NtCOMT1 may represent a solution to cope with the increasing drought stress in tobacco production in Yunnan province.
引用
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页数:10
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