SpeedScreen: the "missing link" between genomics and lead discovery

被引：44

作者：

Zehender, H ^{[1
]}

Le Goff, F

Lehmann, N

Filipuzzi, I

Mayr, LM

机构：

[1] Novartis Inst Biomed Res, Discovery Technol, Basel Screening Operat, WSJ350 208, CH-4002 Basel, Switzerland

[2] Novartis Inst Biomed Res, Discovery Technol, Basel Screening Operat, WSJ350 209, CH-4002 Basel, Switzerland

[3] Novartis Inst Biomed Res, Discovery Technol, Basel Screening Operat, WSJ350 114, CH-4002 Basel, Switzerland

[4] Novartis Inst Biomed Res, Discovery Technol, Basel Screening Operat, WSJ350 102, CH-4002 Basel, Switzerland

来源：

JOURNAL OF BIOMOLECULAR SCREENING | 2004年 / 9卷 / 06期

关键词：

affinity selection; mass spectrometry; high-throughput screening; orphan targets; nontractable targets; chemical genomics;

D O I：

10.1177/1087057104267605

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

SpeedScreen is a novel, label-free, in-solution, affinity-based selection methodology for high-throughput screening (HTS) developed at Novartis Pharma. The SpeedScreen protocol comprises in-solution affinity selection, followed by size exclusion chromatography in combination with microbore-liquid-chromatography/electrospray-ionization mass spectrometry (micro-LC/ESI-MS). The authors describe the basic concept behind assay development, HTS, and data analysis with the SpeedScreen technology. Advantages and limitations of SpeedScreen compared to alternative screening technologies are discussed, and an example is given from a SpeedScreen campaign applying this innovative affinity selection concept in HTS.

引用

页码：498 / 505

页数：8

共 5 条

[1] The ubiquitin system [J].

Hershko, A ;

Ciechanover, A .

ANNUAL REVIEW OF BIOCHEMISTRY, 1998, 67 :425-479

[2] INSTRUMENTATION, APPLICATIONS, AND ENERGY DEPOSITION IN QUADRUPOLE ION-TRAP TANDEM MASS-SPECTROMETRY [J].

LOURIS, JN ;

COOKS, RG ;

SYKA, JEP ;

KELLEY, PE ;

STAFFORD, GC ;

TODD, JFJ .

ANALYTICAL CHEMISTRY, 1987, 59 (13) :1677-1685

[3] SpeedScreen: label-free liquid chromatography-mass spectrometry-based high-throughput screening for the discovery of orphan protein ligands [J].

Muckenschnabel, I ;

Falchetto, R ;

Mayr, LM ;

Filipuzzi, I .

ANALYTICAL BIOCHEMISTRY, 2004, 324 (02) :241-249

[4] Staurosporine-induced conformational changes of cAMP-dependent protein kinase catalytic subunit explain inhibitory potential [J].

Prade, L ;

Engh, RA ;

Girod, A ;

Kinzel, V ;

Huber, R ;

Bossemeyer, D .

STRUCTURE, 1997, 5 (12) :1627-1637

[5] The sequence of the human genome [J].

Venter, JC ;

Adams, MD ;

Myers, EW ;

Li, PW ;

Mural, RJ ;

Sutton, GG ;

Smith, HO ;

Yandell, M ;

Evans, CA ;

Holt, RA ;

Gocayne, JD ;

Amanatides, P ;

Ballew, RM ;

Huson, DH ;

Wortman, JR ;

Zhang, Q ;

Kodira, CD ;

Zheng, XQH ;

Chen, L ;

Skupski, M ;

Subramanian, G ;

Thomas, PD ;

Zhang, JH ;

Miklos, GLG ;

Nelson, C ;

Broder, S ;

Clark, AG ;

Nadeau, C ;

McKusick, VA ;

Zinder, N ;

Levine, AJ ;

Roberts, RJ ;

Simon, M ;

Slayman, C ;

Hunkapiller, M ;

Bolanos, R ;

Delcher, A ;

Dew, I ;

Fasulo, D ;

Flanigan, M ;

Florea, L ;

Halpern, A ;

Hannenhalli, S ;

Kravitz, S ;

Levy, S ;

Mobarry, C ;

Reinert, K ;

Remington, K ;

Abu-Threideh, J ;

Beasley, E .

SCIENCE, 2001, 291 (5507) :1304-+

← 1 →