Rapid Determination of Volatile Compounds in Gymnotheca involucrata Pei. by MAE-HS-SPME Followed by GC-MS

Microwave-assisted extraction (MAE) and headspace solid-phase microextraction (HS-SPME) followed by gas chromatography-mass spectrometry (GC-MS) was developed for the rapid determination of the volatile chemical compositions of Gymnotheca involucrata Pei. The experimental parameters of the MAE-HS-SPME were optimized by orthogonal experimental design. The results indicated that the optimal experimental parameters for determination of the volatile constituents of Gymnotheca involucrata Pei. were: sample weight 2.0 g, microwave power of 400 W and irradiation time of 3.0 min. Employing these conditions, 106 volatile constituents in the dry stems and 103 volatile constituents in the dry leaves of Gymnotheca involucrata Pei. were separated and identified, amounting to 93.58 and 98.53% of the total peak areas, respectively. The major components found in the oil extracted from the stems and leaves were nerolidol (16.57, 17.50%), sabinene (3.35, 3.65%), cis, cis, cis-1,1,4,8-tetramethyl-4,7,10-cycloundecatriene (4.64, 5.03%), alpha-copaene-8-ol (6.01, 6.33%) and phytol (2.92, 3.03%), respectively. Relative standard deviation (RSD) values of < 9.0% showed that the MAE-HS-SPME method followed by GC-MS has good precision. The experimental results demonstrated that this solvent-free method is simple, time-efficient, and is a potentially suitable analytical tool for the determination of volatile compounds from vegetative and other materials.