Transcriptome analysis of quorum-sensing regulation and virulence factor expression in Pseudomonas aeruginosa

被引：66
作者：
Wagner, VE [1 ]
Gillis, RJ [1 ]
Iglewski, BH [1 ]
机构：
[1] Univ Rochester, Sch Med & Dent, Dept Microbiol & Immunol, Rochester, NY 14642 USA
来源：
VACCINE | 2004年 / 22卷
关键词：
Pseudomonas aeruginosa; quorum-sensing; virulence;
D O I：
10.1016/j.vaccine.2004.08.011
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
The opportunistic pathogen Pseudomonas aeruginosa possesses two well-studied quorum-sensing (QS) systems (las and rhl) that are important in the production of virulence factors, antibiotic sensitivity, and biofilm development. High-density oligonucleotide microarrays were used to further characterize the las QS system and to investigate the effect of environment (planktonic or biofilm mode of growth, absence or presence of oxygen) and nutritional conditions on detection of transcripts encoding QS-regulated virulence factors. Transcriptome results indicate that the QS system is far more complex than previously proposed. Interestingly, we found that many QS-regulated genes encoding virulence products were expressed in all conditions investigated. (C) 2004 Elsevier Ltd. All rights reserved.
引用
收藏
页码：S15 / S20
页数：6
相关论文
共 38 条
[1]  
BAINBRIDGE T, 1989, J LAB CLIN MED, V114, P728
[2]   Regulation of the xcp secretion pathway by multiple quorum-sensing modulons in Pseudomonas aeruginosa [J].
ChaponHerve, V ;
Akrim, M ;
Latifi, A ;
Williams, P ;
Lazdunski, A ;
Bally, M .
MOLECULAR MICROBIOLOGY, 1997, 24 (06) :1169-1178
[3]   Bacterial biofilms: A common cause of persistent infections [J].
Costerton, JW ;
Stewart, PS ;
Greenberg, EP .
SCIENCE, 1999, 284 (5418) :1318-1322
[4]   The involvement of cell-to-cell signals in the development of a bacterial biofilm [J].
Davies, DG ;
Parsek, MR ;
Pearson, JP ;
Iglewski, BH ;
Costerton, JW ;
Greenberg, EP .
SCIENCE, 1998, 280 (5361) :295-298
[5]   Bacterial quorum sensing in pathogenic relationships [J].
de Kievit, TR ;
Iglewski, BH .
INFECTION AND IMMUNITY, 2000, 68 (09) :4839-4849
[6]   Quorum-sensing genes in Pseudomonas aeruginosa biofilms:: Their role and expression patterns [J].
De Kievit, TR ;
Gillis, R ;
Marx, S ;
Brown, C ;
Iglewski, BH .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2001, 67 (04) :1865-1873
[7]  
De Kievit TR, 1999, METHOD ENZYMOL, V310, P117
[8]   LASR OF PSEUDOMONAS-AERUGINOSA IS A TRANSCRIPTIONAL ACTIVATOR OF THE ALKALINE PROTEASE GENE (APR) AND AN ENHANCER OF EXOTOXIN-A EXPRESSION [J].
GAMBELLO, MJ ;
KAYE, S ;
IGLEWSKI, BH .
INFECTION AND IMMUNITY, 1993, 61 (04) :1180-1184
[9]   CLONING AND CHARACTERIZATION OF THE PSEUDOMONAS-AERUGINOSA LASR GENE, A TRANSCRIPTIONAL ACTIVATOR OF ELASTASE EXPRESSION [J].
GAMBELLO, MJ ;
IGLEWSKI, BH .
JOURNAL OF BACTERIOLOGY, 1991, 173 (09) :3000-3009
[10]   Roles of Pseudomonas aeruginosa las and rhl quorum-sensing systems in control of twitching motility [J].
Glessner, A ;
Smith, RS ;
Iglewski, BH ;
Robinson, JB .
JOURNAL OF BACTERIOLOGY, 1999, 181 (05) :1623-1629
1234