Intein-mediated purification of a recombinantly expressed peptide

被引：12
|
作者：
Pezza, JA
Allen, KN
Tolan, DR
机构：
[1] Boston Univ, Dept Biol, Boston, MA 02215 USA
[2] Boston Univ, Sch Med, Dept Physiol & Biophys, Boston, MA 02118 USA
来源：
CHEMICAL COMMUNICATIONS | 2004年 / 21期
关键词：
D O I：
10.1039/b407924h
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
A 26 amino acid peptide has successfully been purified via recombinant expression as an intein fusion protein accompanied by cleavage without the need for any exogenous proteases or cofactors, thus offering a practical, inexpensive approach to produce isotopically labelled peptides.
引用
收藏
页码：2412 / 2413
页数：2
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