ZnO nanocrystals shuttled by extracellular vesicles as effective Trojan nano-horses against cancer cells

被引：46

作者：

Dumontel, Bianca ^{[1
]}

Susa, Francesca ^{[1
]}

Limongi, Tania ^{[1
]}

Canta, Marta ^{[1
]}

Racca, Luisa ^{[1
]}

Chiodoni, Angelica ^{[2
]}

Garino, Nadia ^{[1
,2
]}

Chiabotto, Giulia ^{[3
,4
]}

Centomo, Maria L. ^{[3
,5
]}

Pignochino, Ymera ^{[3
,5
]}

Cauda, Valentina ^{[1
]}

机构：

[1] Politecn Torino, Dept Appl Sci & Technol, Corso Duca Abruzzi 24, I-10129 Turin, Italy

[2] Ist Italiano Tecnol, Ctr Sustainable Future Technol CSFT POLITO, Via Livorno 60, I-10144 Turin, Italy

[3] IRCCS, FPO, Sarcoma Unit, Div Med Oncol,Candiolo Canc Inst, Str Provle 142,Km 3-95, I-10060 Candiolo, TO, Italy

[4] Univ Torino, Dept Med Sci, I-10126 Turin, Italy

[5] Univ Torino, Dept Oncol, Sr Provle 142,Km 3-95, I-0060 Candiolo, TO, Italy

来源：

NANOMEDICINE | 2019年 / 14卷 / 21期

基金：

欧洲研究理事会;

关键词：

biomimetics; colloidal stability; cytotoxicity; extracellular vesicles; nanocrystals; zinc oxide; ZINC-OXIDE NANOCRYSTALS; MESOPOROUS SILICA NANOPARTICLES; CELLULAR UPTAKE; DRUG-DELIVERY; EXOSOMES; TOXICITY; CARRIERS; DESIGN;

D O I：

10.2217/nnm-2019-0231

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Aim: The effective application of nanoparticles in cancer theranostics is jeopardized by their aggregation in biological media, rapid degradation and clearance. The design of biomimetic nanoconstructs with enhanced colloidal stability and non-immunogenicity is therefore essential. We propose naturally stable cell-derived extracellular vesicles to encapsulate zinc oxide (ZnO) nanocrystals as efficacious nanodrugs, to obtain highly biomimetic and stable Trojan nano-horses (TNHs). Materials & methods: Coupling efficiency, biostability, cellular cytotoxicity and internalization were tested. Results:In vitro studies showed a high internalization of TNHs into cancer cells and efficient cytotoxic activity thanks to ZnO intracellular release. Conclusion: TNHs represent an efficient biomimetic platform for future nanotheranostic applications, with biomimetic extracellular vesicle-lipid envelope, facilitated ZnO cellular uptake and potential therapeutic implications. [GRAPHICS]

引用

页码：2815 / 2833

页数：19

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