Chemically Induced Cardiomyogenesis of Mouse Embryonic Stem Cells

被引：15

作者：

Berkessel, Albrecht ^{[1
]}

Seelig, Bianca ^{[1
]}

Schwengberg, Silke ^{[2
]}

Hescheler, Juergen ^{[3
,4
]}

Sachinidis, Agapios ^{[3
,4
]}

机构：

[1] Univ Cologne, Dept Chem, D-50939 Cologne, Germany

[2] Axiogenesis AG, D-50829 Cologne, Germany

[3] Univ Cologne CMMC, Ctr Physiol & Pathophysiol, Inst Neurophysiol, D-50931 Cologne, Germany

[4] Univ Cologne CMMC, Ctr Mol Med, D-50931 Cologne, Germany

来源：

CHEMBIOCHEM | 2010年 / 11卷 / 02期

关键词：

cardiomyogenesis; cells; cinchona alkaloids; screening; stem cells; DYNAMIC KINETIC RESOLUTION; DIFFERENTIATION; ORGANOCATALYSTS; CARDIOMYOCYTES; IDENTIFICATION; MOLECULES; SURVIVAL; ATRIAL;

D O I：

10.1002/cbic.200900345

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A transgenic murine embryonic stem (ES) cell lineage expressing enhanced green fluorescent protein (EGFP) under the control of alpha-myosine heavy chain (alpha-MHC) promoter (p alpha-MHC-EGFP) was used to investigate the effects of (thio)urea and cinchona alkaloid derivatives on cardiomyogenesis. The screening of the compounds yielded cardiomyogenesis inducing substances with good (IV-5, V-4) to very good activities (II-16, IV-8), as determined by a 50 to 80% increase in the EGFP fluorescence compared to untreated cells. Time-dependent screening approaches in which compounds were added at different developmental stages of the ES cells appeared to be of limited suitability for the identification of potential cellular targets.

引用

页码：208 / 217

页数：10

共 24 条

[1] Highly efficient dynamic kinetic resolution of azlactones by urea-based bifunctional organocatalysts [J].

Berkessel, A ;

Cleemann, F ;

Mukherjee, S ;

Müller, TN ;

Lex, J .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2005, 44 (05) :807-811

[2] Second-generation organocatalysts for the highly enantioselective dynamic kinetic resolution of azlactones [J].

Berkessel, A ;

Mukherjee, S ;

Cleemann, F ;

Müller, TN ;

Lex, J .

CHEMICAL COMMUNICATIONS, 2005, (14) :1898-1900

[3]

Berkessel A, 2005, ASYMMETRIC ORGANOCATALYSIS: FROM BIOMIMETIC CONCEPTS TO APPLICATIONS IN ASYMMETRIC SYNTHESIS, P1, DOI 10.1002/3527604677

[4]

Berkessel A., 2005, ANGEW CHEM, V117, P817

[5] Highly enantioselective epoxidation of 2-methylnaphthoquinone (vitamin K3) mediated by new Cinchona alkaloid phase-transfer catalysts [J].

Berkessel, Albrecht ;

Guixa, Maria ;

Schmidt, Friederike ;

Neudoerfl, Joerg M. ;

Lex, Johann .

CHEMISTRY-A EUROPEAN JOURNAL, 2007, 13 (16) :4483-4498

[6] Structural optimization of thiourea-based bifunctional organocatalysts for the highly enantioselective dynamic kinetic resolution of azlactones [J].

Berkessel, Albrecht ;

Mukherjee, Santanu ;

Mueller, Thomas N. ;

Cleemann, Felix ;

Roland, Katrin ;

Brandenburg, Marc ;

Neudoerfl, Joerg-M. ;

Lex, Johann .

ORGANIC & BIOMOLECULAR CHEMISTRY, 2006, 4 (23) :4319-4330

[7] Asymmetric Morita-Baylis-Hillman reaction catalyzed by isophoronediamine-derived bis(thio)urea organocatalysts [J].

Berkessel, Albrecht ;

Roland, Katrin ;

Neudoerfl, Jorg M. .

ORGANIC LETTERS, 2006, 8 (19) :4195-4198

[8] Differentiation of pluripotent embryonic stem cells into cardiomyocytes [J].

Boheler, KR ;

Czyz, J ;

Tweedie, D ;

Yang, HT ;

Anisimov, SV ;

Wobus, AM .

CIRCULATION RESEARCH, 2002, 91 (03) :189-201

[9]

BOHM HJ, 1996, WIRKSTOFFDESIGN, V159, P437

[10] A role for chemistry in stem cell biology [J].

Ding, S ;

Schultz, PG .

NATURE BIOTECHNOLOGY, 2004, 22 (07) :833-840

← 1 2 3 →