Structural Insights into Polyhydroxyalkanoates Biosynthesis

被引：89
作者：
Sagong, Hye-Young [1 ,2 ]
Son, Hyeoncheol Francis [1 ,2 ]
Choi, So Young [3 ,4 ]
Lee, Sang Yup [3 ,4 ]
Kim, Kyung-Jin [1 ,2 ]
机构：
[1] Kyungpook Natl Univ, Sch Life Sci, KNU Creat BioRes Grp, Daegu 41566, South Korea
[2] Kyungpook Natl Univ, KNU Inst Microorganisms, Daegu 41566, South Korea
[3] Korea Adv Inst Sci & Technol, Metabol & Biomol Engn Natl Res Lab, Dept Chem & Biomol Engn, Plus Program BK21,BioProc Engn Res Ctr,Ctr Syst &, Daejeon 34141, South Korea
[4] Korea Adv Inst Sci & Technol, Inst BioCentury, Daejeon 34141, South Korea
来源：
TRENDS IN BIOCHEMICAL SCIENCES | 2018年 / 43卷 / 10期
基金：
新加坡国家研究基金会;
关键词：
RALSTONIA-EUTROPHA H16; TRANSMISSION ELECTRON-MICROSCOPY; GRANULE-ASSOCIATED PROTEINS; ALCALIGENES-EUTROPHUS; CRYSTAL-STRUCTURE; POLYHYDROXYBUTYRATE SYNTHASE; PSEUDOMONAS-AERUGINOSA; ESCHERICHIA-COLI; PHA SYNTHASE; BACILLUS-MEGATERIUM;
D O I：
10.1016/j.tibs.2018.08.005
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Polyhydroxyalkanoates (PHAs) are diverse biopolyesters produced by numerous microorganisms and have attracted much attention as a substitute for petroleum-based polymers. Despite several decades of study, the detailed molecular mechanisms of PHA biosynthesis have remained unknown due to the lack of structural information on the key PHA biosynthetic enzyme PHA synthase. The recently determined crystal structure of PHA synthase, together with the structures of acetyl-coenzyme A (CoA) acetyltransferase and reductase, have changed this situation. Structural and biochemical studies provided important clues for the molecular mechanisms of each enzyme as well as the overall mechanism of PHA biosynthesis from acetyl-CoA. This new information and knowledge is expected to facilitate production of designed novel PHAs and also enhanced production of PHAs.
引用
收藏
页码：790 / 805
页数：16
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