Identification of FAM3D as a new endogenous chemotaxis agonist for the formyl peptide receptors

被引：35

作者：

Peng, Xinjian ^{[1
,2
]}

Xu, Enquan ^{[1
,2
]}

Liang, Weiwei ^{[1
,2
]}

Pei, Xiaolei ^{[1
,2
]}

Chen, Dixin ^{[1
,2
]}

Zheng, Danfeng ^{[1
,2
]}

Zhang, Yang ^{[1
,2
]}

Zheng, Can ^{[1
,2
]}

Wang, Pingzhang ^{[1
,2
,3
]}

She, Shaoping ^{[1
,2
]}

Zhang, Yan ^{[1
,2
]}

Ma, Jing ^{[1
,2
]}

Mo, Xiaoning ^{[3
]}

Zhang, Yingmei ^{[1
,2
,3
]}

Ma, Dalong ^{[1
,2
,3
]}

Wang, Ying ^{[1
,2
,3
]}

机构：

[1] Peking Univ, Hlth Sci Ctr, Sch Basic Med Sci, Dept Immunol, Beijing 100191, Peoples R China

[2] Peking Univ, Hlth Sci Ctr, Key Lab Med Immunol, Minist Hlth, Beijing 100191, Peoples R China

[3] Peking Univ, Ctr Human Dis Genom, Beijing 100191, Peoples R China

来源：

JOURNAL OF CELL SCIENCE | 2016年 / 129卷 / 09期

基金：

中国国家自然科学基金; 高等学校博士学科点专项科研基金;

关键词：

FAM3D; FPR1; FPR2; Neutrophils; Colitis; PROTEIN-COUPLED RECEPTORS; CHEMOKINE RECEPTORS; CHEMOATTRACTANT PROTEIN; CC-CHEMOKINE; LIGAND; RECONSTITUTION; INHIBITION; EXPRESSION; MEMBERS; KINASE;

D O I：

10.1242/jcs.183053

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The family with sequence similarity 3 (FAM3) gene family is a cytokine-like gene family with four members FAM3A, FAM3B, FAM3C and FAM3D. In this study, we found that FAM3D strongly chemoattracted human peripheral blood neutrophils and monocytes. To identify the FAM3D receptor, we used chemotaxis, receptor internalization, Ca2+ flux and radioligand-binding assays in FAM3D-stimulated HEK293 cells that transiently expressed formyl peptide receptor (FPR) 1 or FPR2 to show that FAM3D was a high affinity ligand of these receptors, both of which were highly expressed on the surface of neutrophils, and monocytes and macrophages. After being injected into the mouse peritoneal cavity, FAM3D chemoattracted CD11b+ Ly6G+ neutrophils in a short time. In response to FAM3D stimulation, phosphorylated ERK1/2 and phosphorylated p38 MAPK family proteins were upregulated in the mouse neutrophils, and this increase was inhibited upon treatment with an inhibitor of FPR1 or FPR2. FAM3D has been reported to be constitutively expressed in the gastrointestinal tract. We found that FAM3D expression increased significantly during colitis induced by dextran sulfate sodium. Taken together, we propose that FAM3D plays a role in gastrointestinal homeostasis and inflammation through its receptors FPR1 and FPR2.

引用

页码：1831 / 1842

页数：12

共 42 条

[1] Annexin A1 regulates intestinal mucosal injury, inflammation, and repair [J].