Characterization of Gymnodinium mikimotoi (Dinophyceae) nuclei and identification of the major histone-like protein, HGm

被引：11
|
作者：
Wargo, MJ [1 ]
Rizzo, PJ [1 ]
机构：
[1] Texas A&M Univ, Dept Biol, College Stn, TX 77843 USA
来源：
JOURNAL OF PHYCOLOGY | 2000年 / 36卷 / 03期
关键词：
dinoflagellate; Dinophyceae; histone-like protein; nuclear isolation; red tide;
D O I：
10.1046/j.1529-8817.2000.99122.x
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
A simple and rapid method for isolation of nuclei from Gymnodinium mikimotoi Miyake et Kominami ex Oda is described along with chemical characterization of the nuclei, The isolated nuclei were completely free of the hole cells, 99.96% free of cytoplasmic contamination, and were collected with a yield of 40% from harvested whole cells, Each nucleus contained 47 pg of DNA and the ratio of DNA to acid-soluble proteins to acid-insoluble proteins was 1:0,25:1,21, respectively. SDS electrophoresis of acid-extracted proteins showed one histone-like protein, which we termed HGm, with an apparent molecular mass of 12 kDa. V8 protease digestion analysis of HGm, the histone-like protein from Crypthecodinium cohnii (HCc), and two histone-like proteins from Gymnodinium dorsum, showed that the HGm digestion pattern was more similar to that of HCc than to that of either of the G, dorsum histone-like proteins.
引用
收藏
页码：584 / 589
页数：6
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