Characterization of Gymnodinium mikimotoi (Dinophyceae) nuclei and identification of the major histone-like protein, HGm

被引：11

作者：

Wargo, MJ ^{[1
]}

Rizzo, PJ ^{[1
]}

机构：

[1] Texas A&M Univ, Dept Biol, College Stn, TX 77843 USA

来源：

JOURNAL OF PHYCOLOGY | 2000年 / 36卷 / 03期

关键词：

dinoflagellate; Dinophyceae; histone-like protein; nuclear isolation; red tide;

D O I：

10.1046/j.1529-8817.2000.99122.x

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

A simple and rapid method for isolation of nuclei from Gymnodinium mikimotoi Miyake et Kominami ex Oda is described along with chemical characterization of the nuclei, The isolated nuclei were completely free of the hole cells, 99.96% free of cytoplasmic contamination, and were collected with a yield of 40% from harvested whole cells, Each nucleus contained 47 pg of DNA and the ratio of DNA to acid-soluble proteins to acid-insoluble proteins was 1:0,25:1,21, respectively. SDS electrophoresis of acid-extracted proteins showed one histone-like protein, which we termed HGm, with an apparent molecular mass of 12 kDa. V8 protease digestion analysis of HGm, the histone-like protein from Crypthecodinium cohnii (HCc), and two histone-like proteins from Gymnodinium dorsum, showed that the HGm digestion pattern was more similar to that of HCc than to that of either of the G, dorsum histone-like proteins.

引用

页码：584 / 589

页数：6

共 27 条

[11] Oligomeric structures of the phage O29 histone-like protein p6
Abril, AM
Marco, S
Carrascosa, JL
Salas, M
Hermoso, JM
JOURNAL OF MOLECULAR BIOLOGY, 1999, 292 (03) : 581 - 588
[12] Mycobacterial laminin-binding histone-like protein mediates collagen-dependent cytoadherence
Dias, Andre Alves
Raze, Dominique
de Lima, Cristiana Soares
Marques, Maria Angela de Melo
Drobecq, Herve
Debrie, Anne-Sophie
Ribeiro-Guimaraes, Michelle Lopes
Biet, Franck
Vidal Pessolani, Maria Cristina
MEMORIAS DO INSTITUTO OSWALDO CRUZ, 2012, 107 : 174 - +
[13] DNA binding ability of histone-like protein HPhA is negatively affected by interaction with Pb2+
Wang, Yanyan
Wang, Ye
Zhan, Yang
Zhang, Jinrui
Liang, Weiguo
Fang, Xuexun
Yu, Dahai
Feng, Yan
BIOMETALS, 2015, 28 (01) : 207 - 217
[14] A Histone-Like Protein Induces Plasmid DNA to Form Liquid Crystals in Vitro and Gene Compaction in Vivo
Sun, Shiyong
Liu, Mingxue
Dong, Faqin
Fan, Shenglan
Yao, Yanchen
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2013, 14 (12): : 23842 - 23857
[15] A histone-like protein of Helicobacter pylori protects DNA from stress damage and aids host colonization
Wang, Ge
Lo, Leja F.
Maier, Robert J.
DNA REPAIR, 2012, 11 (09) : 733 - 740
[16] Cloning and sequencing of the hup gene encoding the histone-like protein HS1 of Streptomyces lividans
Yokoyama, E
Doi, K
Ogata, S
BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION, 1997, 1353 (02): : 103 - 106
[17] DNA binding ability of histone-like protein HPhA is negatively affected by interaction with Pb2+
Yanyan Wang
Ye Wang
Yang Zhan
Jinrui Zhang
Weiguo Liang
Xuexun Fang
Dahai Yu
Yan Feng
BioMetals, 2015, 28 : 207 - 217
[18] Detection of the single-stranded DNA of Streptomyces plasmid pSA1.1 and a binding histone-like protein
Yokoyama, E
Doi, K
Kimura, M
Ogata, S
FEMS MICROBIOLOGY LETTERS, 1996, 138 (2-3) : 197 - 200
[19] Structural plasticity and thermal stability of the histone-like protein from Spiroplasma melliferum are due to phenylalanine insertions into the conservative scaffold
Timofeev, Vladimir I.
Altukhov, Dmitry A.
Talyzina, Anna A.
Agapova, Yulia K.
Vlaskina, Anna V.
Korzhenevskiy, Dmitry A.
Kleymenov, Sergey Yu.
Bocharov, Eduard V.
Rakitina, Tatiana V.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS, 2018, 36 (16) : 4392 - 4404
[20] A gene, han1A, encoding an archaeal histone-like protein from the Thermococcus species AN1: Homology with eukaryal histone consensus sequences and the implications for delineation of the histone fold
Ronimus, RS
Musgrave, DR
BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION, 1996, 1307 (01): : 1 - 7

← 1 2 3 →