Measurement of refractive index distribution of biotissues by scanning focused refractive index microscopy

被引：0
作者：
Sun, Teng-Qian [1 ]
Ye, Qing [1 ]
Wang, Xiao-Wan [1 ]
Wang, Jin [1 ]
Deng, Zhi-Chao [1 ]
Mei, Jian-Chun [2 ]
Zhou, Wen-Yuan [2 ]
Zhang, Chun-Ping [1 ]
Tian, Jian-Guo [1 ]
机构：
[1] Nankai Univ, Sch Phys, Key Lab Weak Light Nonlinear Photon, Minist Educ, Tianjin 300071, Peoples R China
[2] Nankai Univ, Adv Technol Inst, Tianjin 300071, Peoples R China
来源：
OPTICS IN HEALTH CARE AND BIOMEDICAL OPTICS VI | 2014年 / 9268卷
基金：
对外科技合作项目（国际科技项目）;
关键词：
scanning focused refractive index microscopy; biological tissues; refractive index imaging; DIGITAL HOLOGRAPHIC MICROSCOPY; LIVING CELLS;
D O I：
10.1117/12.2072026
中图分类号：
O43 [光学];
学科分类号：
070207 ; 0803 ;
摘要：
We adapt the improved scanning focused refractive-index microscopy (SFRIM) technique to the quantitative study of biological tissues. Delicate refractive index (RI) imaging of a porcine muscle tissue is obtained in a reflection mode. Some modifications are made to the SFRIM for better two dimension (2-D) observation of the tissues. The RI accuracy is 0.002. The central spatial resolution of SFRIM achieves 1 mu m, smaller than the size of the focal spot. Our method is free from signal distortion. The experimental result demonstrates that SFRIM is a potential technique in a wide field of biomedical research.
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页数：7
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