Protocol A T-cell-based immunogenicity protocol for evaluating human antigen-specific responses

被引：20

作者：

Bozkus, Cansu Cimen ^{[1
]}

Blazquez, Ana Belen ^{[1
,2
]}

Enokida, Tomohiro ^{[1
,3
]}

Bhardwaj, Nina ^{[1
]}

机构：

[1] Icahn Sch Med Mt Sinai, Dept Hematol & Med Oncol, New York, NY 10127 USA

[2] Bristol Myers Squibb, Global Biol Specimen & Imaging Management GBS&IM, Princeton, NJ 08540 USA

[3] Natl Canc Ctr, Res Inst, Exploratory Oncol Res & Clin Trial Ctr EPOC, Div Canc Immunol, Chiba, Tokyo, Japan

来源：

STAR PROTOCOLS | 2021年 / 2卷 / 03期

关键词：

DENDRITIC CELLS; DIFFERENTIATION;

D O I：

10.1016/j.xpro.2021.100758

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Determining the antigen specificities of the endogenous T-cell repertoire is important for screening naturally occurring or therapy-induced T-cell immunity and may help identify novel targets for T-cell-based therapies. Here, we describe a rapid, sensitive, and high-throughput protocol for expanding antigen-specific T cells from human peripheral blood mononuclear cells in vitro following peptide stimulation and detecting antigen-specific effector cytokine formation by flow cytometry. Our approach can be applied to examining specific T-cell subsets from various tissues. For complete details on the use and execution of this protocol, please refer to Roudko et al. (2020) and Cimen Bozkus et al. (2019).

引用

页数：19