Silver nanospheres are cytotoxic and genotoxic to fish cells

被引：163

作者：

Wise, John Pierce, Sr. ^{[1
,2
,3
,4
]}

Goodale, Britton C. ^{[2
]}

Wise, Sandra S. ^{[2
,3
,4
]}

Craig, Gary A. ^{[5
,6
]}

Pongan, Adam F. ^{[5
,6
]}

Walter, Ronald B. ^{[7
]}

Thompson, W. Douglas ^{[3
]}

Ng, Ah-Kau ^{[3
]}

Aboueissa, AbouEl-Makarim ^{[8
]}

Mitani, Hiroshi ^{[9
]}

Spalding, Mark J. ^{[10
]}

Mason, Michael D. ^{[5
,6
]}

机构：

[1] Univ So Maine, Lab Environm & Genet Toxicol, Maine Ctr Toxicol & Environm Hlth, Portland, ME 04104 USA

[2] Univ So Maine, Wise Lab Environm & Genet Toxicol, Portland, ME 04104 USA

[3] Univ So Maine, Dept Appl Sci Med, Portland, ME 04104 USA

[4] Ocean Alliance, Lincoln, MA 01773 USA

[5] Univ Maine, Dept Chem & Biol Engn, Orono, ME 04469 USA

[6] Univ Maine, Inst Mol Biophys, Orono, ME 04469 USA

[7] Texas State Univ, Xiphophorus Genet Stock Ctr, Mol Biosci Res Grp, San Marcos, TX 78666 USA

[8] Univ So Maine, Dept Math & Stat, Portland, ME 04104 USA

[9] Univ Tokyo, Grad Sch Frontier Sci, Dept Integrated Biosci, Chiba 2778562, Japan

[10] Ocean Fdn, Washington, DC 20036 USA

来源：

AQUATIC TOXICOLOGY | 2010年 / 97卷 / 01期

关键词：

Silver; Nanoparticle; Genotoxicity; Medaka; WALL CARBON NANOTUBES; HEXAVALENT CHROMIUM; PULMONARY TOXICITY; DNA-DAMAGE; NANOPARTICLES; MEDAKA; REDUCTION; PARTICLES; EXPOSURE;

D O I：

10.1016/j.aquatox.2009.11.016

中图分类号：

Q17 [水生生物学];

学科分类号：

071004 ;

摘要：

Nanoparticles are being widely investigated for a range of applications due to their unique physical properties. For example, silver nanoparticles are used in commercial products for their antibacterial and antifungal properties. Some of these products are likely to result in silver nanoparticles reaching the aquatic environment. As such, nanoparticles pose a health concern for humans and aquatic species. We used a medaka (Oryzias latipes) cell line to investigate the cytotoxicity and genotoxicity of 30 nm diameter silver nanospheres. Treatments of 0.05, 0.3, 0.5, 3 and 5 mu g/cm(2) induced 80, 45.7, 24.3, 1 and 0.1% survival, respectively, in a colony forming assay. Silver nanoparticles also induced chromosomal aberrations and aneuploidy. Treatments of 0, 0.05, 0.1 and 0.3 mu g/cm(2) induced damage in 8, 10.8, 16 and 15.8% of metaphases and 10.8, 15.6, 24 and 24 total aberrations in 100 metaphases, respectively. These data show that silver nanoparticles are cytotoxic and genotoxic to fish cells. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：34 / 41

页数：8

共 50 条

[1] DNA damage response to different surface chemistry of silver nanoparticles in mammalian cells [J].

Ahamed, Maqusood ;

Karns, Michael ;

Goodson, Michael ;

Rowe, John ;

Hussain, Saber M. ;

Schlager, John J. ;

Hong, Yiling .

TOXICOLOGY AND APPLIED PHARMACOLOGY, 2008, 233 (03) :404-410

[2]

[Anonymous], 1950, Experimental Designs

[3]

[Anonymous], PROJ EM NAN CONS PRO

[4] Cellular responses induced by silver nanoparticles:: In vitro studies [J].

Arora, S. ;

Jain, J. ;

Rajwade, J. M. ;

Paknikar, K. M. .

TOXICOLOGY LETTERS, 2008, 179 (02) :93-100

[5] Toxicity of silver nanoparticles in zebrafish models [J].

Asharani, P. V. ;

Wu, Yi Lian ;

Gong, Zhiyuan ;

Valiyaveettil, Suresh .

NANOTECHNOLOGY, 2008, 19 (25)

[6] Cytotoxicity and Genotoxicity of Silver Nanoparticles in Human Cells [J].

AshaRani, P. V. ;

Mun, Grace Low Kah ;

Hande, Manoor Prakash ;

Valiyaveettil, Suresh .

ACS NANO, 2009, 3 (02) :279-290

[7]

Berne B. J., 1975, DYNAMIC LIGHT SCATTE

[8] The use of gold mercaptides for decorative precious metal applications [J].

Bishop, PT .

GOLD BULLETIN, 2002, 35 (03) :89-98

[9] Preclinical safety and pharmacokinetic profile of ferumoxtran-10, an ultrasmall superparamagnetic iron oxide magnetic resonance contrast agent [J].

Bourrinet, P ;

Bengele, HH ;

Bonnemain, B ;

Dencausse, A ;

Idee, JM ;

Jacobs, PM ;

Lewis, JM .

INVESTIGATIVE RADIOLOGY, 2006, 41 (03) :313-324

[10] In vitro cytotoxicity of nanoparticles in mammalian germline stem cells [J].

Braydich-Stolle, L ;

Hussain, S ;

Schlager, JJ ;

Hofmann, MC .

TOXICOLOGICAL SCIENCES, 2005, 88 (02) :412-419

← 1 2 3 4 5 →