Towards an Understanding of Enhanced Biomass Digestibility by In Planta Expression of a Family 5 Glycoside Hydrolase

被引：9
|
作者：
Donohoe, Bryon S. [1 ]
Wei, Hui [1 ]
Mittal, Ashutosh [1 ]
Shollenberger, Todd [1 ]
Lunin, Vladimir V. [1 ]
Himmel, Michael E. [1 ]
Brunecky, Roman [1 ]
机构：
[1] Natl Renewable Energy Lab, Biosci Ctr, 15013 Denver West Pkwy, Golden, CO 80401 USA
来源：
SCIENTIFIC REPORTS | 2017年 / 7卷
关键词：
ACIDOTHERMUS-CELLULOLYTICUS E1; FERULIC ACID ESTERASE; CELL-WALL; IMPROVES SACCHARIFICATION; ENDOGLUCANASE; RECALCITRANCE; TOBACCO;
D O I：
10.1038/s41598-017-04502-1
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
In planta expression of a thermophilic endoglucanase (AcCel5A) reduces recalcitrance by creating voids and other irregularities in cell walls of Arabidopsis thaliana that increase enzyme accessibility without negative impacts on plant growth or cell wall composition. Our results suggest that cellulose beta-1-4 linkages can be cut sparingly in the assembling wall and that these minimal changes, made at the proper time, have an impact on plant cell wall recalcitrance without negative effects on overall plant development.
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页数：9
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